Abstract：
:In this work, a one-step extraction/transesterification process was developed to directly convert wet oil-bearing microalgal biomass of Chlorella vulgaris ESP-31 into biodiesel using immobilized Burkholderia lipase as the catalyst. The microalgal biomass (water content of 86-91%; oil content 14-63%) was pre-treated by sonication to disrupt the cell walls and then directly mixed with methanol and solvent to carry out the enzymatic transesterification. Addition of a sufficient amount of solvent (hexane is most preferable) is required for the direct transesterification of wet microalgal biomass, as a hexane-to-methanol mass ratio of 1.65 was found optimal for the biodiesel conversion. The amount of methanol and hexane required for the direct transesterification process was also found to correlate with the lipid content of the microalga. The biodiesel synthesis process was more efficient and economic when the lipid content of the microalgal biomass was higher. Therefore, using high-lipid-content microalgae as feedstock appears to be desirable.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Tran DT,Chen CL,Chang JSdoi
10.1016/j.biortech.2012.09.101subject
Has Abstractpub_date
2013-05-01 00:00:00pages
213-21eissn
0960-8524issn
1873-2976pii
S0960-8524(12)01451-4journal_volume
135pub_type
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