Abstract：
:This contribution examines the technical feasibility of producing high added value probiotic biomass from deproteinized and non-supplemented milk whey. The kinetics of growth of Lactobacillus casei in deproteinized goat milk whey was analyzed. Experiments in batch, continuous and fed-batch conditions were conducted in a 3 L fully instrumented bioreactor. Final substrate and biomass concentrations, yields and productivities are reported for different culture strategies. A kinetic analysis was conducted to characterize biomass production, product inhibition effects, and substrate consumption rates. Due to the strong product inhibition, fed-batch cultures at high biomass concentration rendered higher productivity (0.45 g L(-1) h(-1)) than batch and continuous cultures (0.11 g L(-1) h(-1)), complete lactose conversions (<1.0 g of lactose/L at the end of each fed-batch cycle), and a product with higher viable cell counts (2 x 10(10) cell/g of freeze-dried product). Based on our result, high-cell density fed-batch strategies are recommended for commercial production of probiotic L. casei biomass.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Aguirre-Ezkauriatza EJ,Aguilar-Yáñez JM,Ramírez-Medrano A,Alvarez MMdoi
10.1016/j.biortech.2009.10.047subject
Has Abstractpub_date
2010-04-01 00:00:00pages
2837-44issue
8eissn
0960-8524issn
1873-2976pii
S0960-8524(09)01423-0journal_volume
101pub_type
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