Abstract：
:In this study, CRISPRi (clustered regularly interspaced short palindromic repeats interference) was used for the first time to regulate expression of exogenously supplied rfp gene as a proof-of-concept, and endogenous PEPC1 gene as a proof-of-function in Chlamydomonas reinhardtii. The efficiency of 94% and stability of 7 generations via CRISPRi mediated gene regulation in C. reinhardtii have been demonstrated by RFP. Gene PEPC1 encoding proteins are essential for controlling the carbon flux that enters the TCA cycle and plays a crucial role in carbon partitioning of substrates in competition with lipid synthesis. All CrPEPC1 down-regulated strains have lower chlorophyll color, but higher biomass concentration and lipid accumulation rate. The present results revealed that CRISPRi based transcriptional silencing was applicable in C. reinhardtii and expanded the way to improve the yield, titer and productivity of microalgae-based products.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Kao PH,Ng ISdoi
10.1016/j.biortech.2017.04.111subject
Has Abstractpub_date
2017-12-01 00:00:00pages
1527-1537issue
Pt Beissn
0960-8524issn
1873-2976pii
S0960-8524(17)30619-3journal_volume
245pub_type
杂志文章abstract：:Microbial oil production from sulphuric acid treated rice straw hydrolysate (SARSH) by Trichosporon fermentans was performed for the first time. Fermentation of SARSH without detoxification gave a poor lipid yield of 1.7 g/l, which was much lower than the result with glucose or xylose as the single carbon source (13.6...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.04.022
更新日期：2009-10-01 00:00:00
abstract：:Nutritive values of the branches from Olea europaea trees cut at 25, 50, 75 or 100 cm distance from the tip were evaluated by determination of the in vitro digestible organic matter (IVDOM), metabolizable energy (ME), net energy lactation (NEL), and presence nutritional and anti-nutritional components. The values of n...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.09.130
更新日期：2012-01-01 00:00:00
abstract：:Bacillus spp. is widely used as the companion bacterium in the two-step biosynthesis of 2-keto-L-gulonic acid (2-KLG), which is the direct precursor in the production of vitamin C by Ketogulonigenium vulgare. To understand the effects of sporulation and spore stability on 2-KLG production, the spo0A and spoVFA deletio...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.12.080
更新日期：2012-03-01 00:00:00
abstract：:Highly efficient simultaneous removal of Cu(II) and tetracycline (TET) from aqueous solution was accomplished by iron and zinc doped sawdust biochar (Fe/Zn-biochar). The mutual effects and inner mechanisms of their adsorption onto Fe/Zn-biochar were systematically investigated via sole and binary systems, sorption iso...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.08.178
更新日期：2017-12-01 00:00:00
abstract：:Two horizontal subsurface flow reed beds of 75 m(2) each, treating dairy parlor effluent and domestic sewage (about 6.5 m(3)/day), were set-up to determine the efficiency of this system in reducing the polluting load in an isolated mountain rural settlement.A total suspended solids value of about 0.70 g/l and chemical...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/s0960-8524(02)00291-2
更新日期：2003-06-01 00:00:00
abstract：:Successful reclamation of saline-alkaline sites may be enhanced by revegetating with species that are tolerant to factors that limit normal plant growth. Boreal woody plants tested in this study have shown promise for use in saline habitats. This study was conducted to assess the effects of amendment treatments (peat,...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2004.07.005
更新日期：2005-05-01 00:00:00
abstract：:In this study, after screening of 9 fungal strains for their ability to produce calcium malate, it was found that Penicillium sclerotiorum K302 among them could produce high-level of calcium malate. Under the optimal conditions, the titer of calcium malate in the supernatant was 88.6 g/l at flask level. During 10-l fe...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.06.051
更新日期：2013-09-01 00:00:00
abstract：:The effect of biochar on the thermophilic digestion of mono-cardboard was investigated. Compared with control group (T0), the maximum rate of biomethane production was significantly improved after the addition of biochar, especially, it has been improved by 40.6% in T1 (0.77 g/gTS sludge) with the methane production o...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.121782
更新日期：2019-10-01 00:00:00
abstract：:The adaptive response of the fungus Blakeslea trispora to the oxidative stress induced by elevated dissolved oxygen concentrations during carotene production was investigated by measuring the specific activities of catalase (CAT) and superoxide dismutase (SOD) and the micromorphology of the fungus using a computerized...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.06.027
更新日期：2011-09-01 00:00:00
abstract：:A cascade process of a rotational drum fermentation system with leachate recirculation from a methanogenic to the acidogenic reactor was constructed to enhance the hydrolysis and acidification of solid organic waste. Using fresh soybean meal as substrates, two process configurations, Cascade process 1 and 2, without a...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2006.08.015
更新日期：2007-08-01 00:00:00
abstract：:Optimum culture conditions which ease the synthesis of a novel exopolysaccharide (EPS) from a potent marine strain Streptococcus phocae was proposed in this study. The strain grows well at 35 °C, pH 7.0 and NaCl (2%) with lactose and yeast extract as best carbon and nitrogen sources. The maximum yield of EPS (11.75 an...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.12.118
更新日期：2011-04-01 00:00:00
abstract：:The accumulation of soluble microbial production (SMP) in an anammox attached film expanded bed (AAFEB) and its effect on the reactor performance were investigated in this study. During the long-term experiment, an extended HRT resulted in the accumulation of SMP and the change of treatment performance. When the SMP i...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.09.129
更新日期：2016-12-01 00:00:00
abstract：:Yeast dehydrogenases and reductases were overexpressed in Saccharomyces cerevisiae D452-2 to detoxify 2-furaldehyde (furfural) and 5-hydroxymethyl furaldehyde (HMF), two potent toxic chemicals present in acid-hydrolyzed cellulosic biomass, and hence improve cell growth and ethanol production. Among those enzymes, alde...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.02.101
更新日期：2011-05-01 00:00:00
abstract：:Contamination of soil and groundwater with mineral oil-based products is among the most common sources of pollution in Nigeria. This study evaluated the distribution of some heavy metals and hydrocarbon content in soil contaminated with waste-lubricating oil (spent oil), and the effectiveness of some abundantly availa...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.05.048
更新日期：2008-05-01 00:00:00
abstract：:Rhizopus oryzae is strictly inhibited by biodiesel-based by-product crude glycerol, which results in low fumaric acid production. In this study, evolutionary engineering was employed to activate the glycerol utilization pathway for fumaric acid production. An evolved strain G80 was selected, which could tolerate and u...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.07.104
更新日期：2015-11-01 00:00:00
abstract：:The main objective of this paper is to find the optimum operating condition to upgrade the EFB-derived pyrolysis oil (bio-oil) to liquid fuel, mainly gasoline using Taguchi Method. From the analysis that has been done, it is found that the optimum operating condition for heterogeneous catalytic cracking process is at ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.05.036
更新日期：2010-11-01 00:00:00
abstract：:Six biomass pretreatment processes to convert switchgrass to fermentable sugars and ultimately to cellulosic ethanol are compared on a consistent basis in this technoeconomic analysis. The six pretreatment processes are ammonia fiber expansion (AFEX), dilute acid (DA), lime, liquid hot water (LHW), soaking in aqueous ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.07.051
更新日期：2011-12-01 00:00:00
abstract：:Photobioreactor is deemed to be one of limiting factors for the commercial application of wastewater treatment based on microalgae cultivation. In this study, a novel Flat-Plate Continuous Open Photobioreactor (FPCO-PBR) was developed to treat piggery biogas slurry. The operation parameters, microbial stability and nu...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.10.057
更新日期：2019-01-01 00:00:00
abstract：:A strain of Saccharomyces cerevisiae, KCCM50549, was found to efficiently ferment the inulin-containing carbohydrates in Jerusalem artichoke without acidic or enzymatic pretreatment prior to fermentation. S. cerevisiae KCCM50549 could utilize almost completely the fructo-oligosaccharides present in Jerusalem artichoke...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.08.044
更新日期：2011-01-01 00:00:00
abstract：:The development of a cost-effective hydrolysis for crude cellulose is an essential part of biorefinery developments. To establish such high solid hydrolysis, a new solid state reactor with static mixing is used. However, concentrations >10% (w/w) cause a rate and yield reduction of enzymatic hydrolysis. By optimizing ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.06.052
更新日期：2014-09-01 00:00:00
abstract：:Notwithstanding many efforts to increase the efficiency of anaerobic digestion at low-temperature (winter) conditions, a cost-effective and efficient method is lacking. This study proposes a low-cost method of low-temperature (<35 °C) anaerobic digestion of wastewater, involving supplementation with granular activated...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.123890
更新日期：2020-11-01 00:00:00
abstract：:Enzymes, especially proteases, have become an important and indispensable part of the processes used by the modern food and feed industry to produce a large and diversified range of products for human and animal consumption. A cysteine protease, used extensively in the food industry, was purified from germinated wheat...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/s0960-8524(03)00193-7
更新日期：2004-02-01 00:00:00
abstract：:Although membrane bioreactor is widely used in wastewater treatment, the problem of membrane fouling remains to be resolved. This paper focused on the influence of mechanical stirring on membrane fouling. Ammonium removal decreased with viscous bulking when stirring rates slowed down. Trans-membrane pressure increased...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.03.143
更新日期：2016-07-01 00:00:00
abstract：:Biogas production has been the focus of many individuals in the developing world; there have been several investigations that focus on improving the production process and product quality. In the developing world the lack of advanced technology and capital has hindered the development of energy production. Renewable e...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2017.12.013
更新日期：2018-02-01 00:00:00
abstract：:Single chambered up-flow membrane-less microbial fuel cell (UFML MFC) was developed to study the feasibility of the bioreactor for decolorization of Acid Orange 7 (AO7) and electricity generation simultaneously. The performance of UFML MFC was evaluated in terms of voltage output, chemical oxygen demand (COD) and colo...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.08.078
更新日期：2015-12-01 00:00:00
abstract：:The combined effect of superficial gas velocity, pH, initial phosphate concentration, and light intensity on cell growth was investigated for the mass production of cyanobacterial cells. The light intensity was manipulated to maintain a specific irradiation rate (q(i)) at a constant level for high cell density culture...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.02.012
更新日期：2008-03-01 00:00:00
abstract：:Pyrolysis of woody biomass, agro-residues and seed was carried out at 500 ± 10 °C in a fixed bed pyrolyser. Bio-oil yield was found varying from 20.5% to 47.5%, whereas the biochar and pyrolysis gas ranged from 27.5% to 40% and 24.5% to 40.5%, respectively. Pyrolysis gas was measured for flame temperature along with C...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.02.006
更新日期：2015-01-01 00:00:00
abstract：:The garbage enzymes produced from preconsumer organic waste containing multi hydrolytic enzyme activity which helps to solubilize the waste activated sludge. The continuous production of garbage enzyme and its scaling up process need a globe optimized condition. In present study the effect of fruit peel composition an...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.12.029
更新日期：2017-02-01 00:00:00
abstract：:Intensive livestock production is connected with a number of environmental effects, including discharges to soils and surface waters and emissions to the atmosphere. In areas with a high livestock density the low availability of nearby arable land, together with the preferred use of chemical fertilizer by arable farme...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.03.003
更新日期：2009-11-01 00:00:00
abstract：:In this investigation, palm empty fruit bunch (EFB) and almond shell (AS) were implemented as two natural catalysts rich in alkali metals, especially potassium, to enhance the reactivity of tire-char through co-gasification process. Co-gasification experiments were conducted at several blending ratios using isothermal...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.03.179
更新日期：2013-06-01 00:00:00
