Abstract:
:Optimum culture conditions which ease the synthesis of a novel exopolysaccharide (EPS) from a potent marine strain Streptococcus phocae was proposed in this study. The strain grows well at 35 °C, pH 7.0 and NaCl (2%) with lactose and yeast extract as best carbon and nitrogen sources. The maximum yield of EPS (11.75 and 12.14 g/L) was obtained in the presence of lactose and yeast extract at a concentration of 20 g/L respectively. EPS was refined by gel filtration chromatography using phenyl Sepharose column which revealed the presence of arabinose, fructose and galactose sugar units with molecular mass about 2.8 × 10(5) Da. Emulsifying and flocculating stability of EPS compared with three commercial hydrocolloids. EPS exhibited better activities which are similar to that of commercial hydrocolloids. Both crude and purified EPS exhibited strong antioxidant potential by quenching hydroxyl and superoxide anion radicals. Antibiofilm activity by inhibition of Gram positive and Gram negative biofilm forming bacteria was evident in our studies. Potential antioxidant activity and biofilm inhibiting property of EPS may lead to the development of novel food grade adjuncts.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Kanmani P,Satish kumar R,Yuvaraj N,Paari KA,Pattukumar V,Arul Vdoi
10.1016/j.biortech.2010.12.118subject
Has Abstractpub_date
2011-04-01 00:00:00pages
4827-33issue
7eissn
0960-8524issn
1873-2976pii
S0960-8524(11)00011-3journal_volume
102pub_type
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