Characterization and optimization of carbohydrate production from an indigenous microalga Chlorella vulgaris FSP-E.

Abstract:

:In this study, three indigenous microalgae isolates were examined for their ability to produce carbohydrates. Among them, Chlorella vulgaris FSP-E displayed relatively high cell growth rate and carbohydrate content. The carbohydrate productivity of C. vulgaris FSP-E was further improved by using engineering strategies. The results show that using an appropriate light intensity and inoculum size could effectively promote cell growth and carbohydrate productivity. Nitrogen starvation triggered the accumulation of carbohydrates in the microalga, achieving a carbohydrate content of 51.3% after 4-day starvation. Under the optimal conditions, the highest biomass and carbohydrate productivity were 1.437 and 0.631 g L(-1) d(-1), respectively. This performance is better than that reported in most related studies. Since glucose accounted for nearly 93% of the carbohydrates accumulated in C. vulgaris FSP-E, the microalga is an excellent feedstock for bioethanol fermentation.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Ho SH,Huang SW,Chen CY,Hasunuma T,Kondo A,Chang JS

doi

10.1016/j.biortech.2012.10.100

subject

Has Abstract

pub_date

2013-05-01 00:00:00

pages

157-65

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(12)01602-1

journal_volume

135

pub_type

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