Abstract:
:The objective of this work is to develop a quick and simple method for the in situ monitoring of sugars in biological cultures. A new technology based on Attenuated Total Reflectance-Fourier Transform Infrared (FT-IR/ATR) spectroscopy in combination with an external light guiding fiber probe was tested, first to build predictive models from solutions of pure sugars, and secondly to use those models to monitor the sugars in the complex culture medium of mixotrophic microalgae. Quantification results from the univariate model were correlated with the total dissolved solids content (R(2)=0.74). A vector normalized multivariate model was used to proportionally quantify the different sugars present in the complex culture medium and showed a predictive accuracy of >90% for sugars representing >20% of the total. This method offers an alternative to conventional sugar monitoring assays and could be used at-line or on-line in commercial scale production systems.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Girard JM,Deschênes JS,Tremblay R,Gagnon Jdoi
10.1016/j.biortech.2013.06.094subject
Has Abstractpub_date
2013-09-01 00:00:00pages
664-8eissn
0960-8524issn
1873-2976pii
S0960-8524(13)01022-5journal_volume
144pub_type
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