Microbial selection pressure is not a prerequisite for granulation: dynamic granulation and microbial community study in a complete mixing bioreactor.

Abstract:

:Microbial selection pressure is traditionally supposed as a prerequisite for aerobic granulation. This work gives a different insight on this issue. Fluorescent microspheres were used to label the flocculent biomass granulation for a period of 47days in a continuous-flow bioreactor. Analysis of the distribution of fluorescent microspheres in granules revealed that the terminal phase of granulation is in a dynamic steady state, where bioflocs detach, collide and aggregate randomly. This revealed that the un-granulated biomass was the result of the dynamic aggregation and breakage, rather than the microbial species unable to be granulated. Furthermore, denaturing gradient gel electrophoresis (DGGE) profile and UPGMA dendrogram results showed similar microbial communities during the granulation. To sum up, microbial selection pressure was not a prerequisite for aerobic granulation from both of the dynamic granulation steps and molecular biology aspects.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Zhou D,Niu S,Xiong Y,Yang Y,Dong S

doi

10.1016/j.biortech.2014.03.001

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

102-8

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(14)00300-9

journal_volume

161

pub_type

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