Sweetpotato vines hydrolysate promotes single cell oils production of Trichosporon fermentans in high-density molasses fermentation.

Abstract:

:This study investigated the co-fermentation of molasses and sweetpotato vine hydrolysate (SVH) by Trichosporon fermentans. T. fermentans showed low lipid accumulation on pure molasses; however, its lipid content increased by 35% when 10% SVH was added. The strong influence of SVH on lipid production was further demonstrated by the result of sensitivity analysis on effects of factors based on an artificial neural network model because the relative importance value of SVH dosage for lipid production was only lower than that of fermentation time. Scanning electron microscope observation and flow cytometry of yeast cells grown in culture with and without SVH showed that less deformation cells were involved in the culture with SVH. The activity of malic enzyme, which plays a key role in fatty acid synthesis, increased from 2.4U/mg to 3.7U/mg after SVH added. All results indicated SVH is a good supplement for lipid fermentation on molasses.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Shen Q,Lin H,Wang Q,Fan X,Yang Y,Zhao Y

doi

10.1016/j.biortech.2014.11.045

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

249-56

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(14)01647-2

journal_volume

176

pub_type

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