Changes in spectroscopic signatures in soluble microbial products of activated sludge under different osmotic stress conditions.

Abstract:

:Spectroscopic techniques were used to examine the subtle changes in soluble microbial products (SMP) of batch activated sludge bioreactors working at different salinities (i.e., 0%, 1%, 3%, and 5% NaCl). The changes in different fluorescent constituent were tracked by excitation-emission matrix combined with parallel factor analysis (EEM-PARAFAC), and the sequential production was further identified by two-dimensional correlation spectroscopy (2D-COS). Greater enrichment of tryptophan-like component and large-sized biopolymer were found in SMP for higher saline bioreactors, suggesting the SMP sources from bound extracellular polymeric substances and excreted intercellular constituents. 2D-COS revealed the opposite sequences of the fluorescence changes in SMP between the low and the high saline bioreactors, following the order of "tyrosine-like > tryptophan-like > humic-like fluorescence" for the latter. This study clarified the dominant mechanisms involved in SMP formation during elevating salinity, which were well supported by the changes in SMP spectroscopic features, microbial activity, and organic degradation rates.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Maqbool T,Cho J,Hur J

doi

10.1016/j.biortech.2018.01.113

subject

Has Abstract

pub_date

2018-05-01 00:00:00

pages

29-38

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(18)30127-5

journal_volume

255

pub_type

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