Abstract：
:An extracellular haloalkaline, thermoactive, solvent stable, SDS-induced serine protease was purified and characterized from an alkali-thermo tolerant strain Bacillus sp. SM2014 isolated from reverse osmosis reject. The enzyme was purified to homogeneity with recovery of 54.4% and purity fold of 64. The purified enzyme was composed of single polypeptide of molecular mass about 71 kDa. The enzyme showed optimum activity at alkaline pH 10 and temperature 60°C. The km and Vmax for the enzyme was 0.57 mg/ml and 445.23 U/ml respectively. The enzyme showed novel catalytic ability at high pH (10), temperature (60°C) and salinity (3M). Moreover, the stability of enzyme in organic solvents (50% v/v) of logP ≥ 2 signified the prospective of this enzyme for peptide synthesis. The compatibility of the enzyme with surfactants and various detergent matrices together with wash performance test confirmed its potential applicability in laundry industry.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Jain D,Pancha I,Mishra SK,Shrivastav A,Mishra Sdoi
10.1016/j.biortech.2011.10.081subject
Has Abstractpub_date
2012-07-01 00:00:00pages
228-36eissn
0960-8524issn
1873-2976pii
S0960-8524(11)01569-0journal_volume
115pub_type
杂志文章abstract：:InCl3, a water-compatible Lewis acid, was used for the conversion of microcrystalline cellulose to produce 5-hydroxymethylfurfural (HMF) in a H2O/THF biphasic system. Addition of NaCl increased the HMF yield significantly but suppressed the levulinic acid (LA) formation. The HMF yield of 39.7% was obtained in 2h at 20...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.09.077
更新日期：2014-11-01 00:00:00
abstract：:Novel biochemical approaches remain to be developed to improve microbial lipid technology. This study demonstrated that sulfate limitation was effective to promote accumulating substantial amounts of intracellular lipid by the oleaginous yeast Rhodosporidium toruloides Y4. When it was cultivated using a medium with an...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.09.033
更新日期：2011-01-01 00:00:00
abstract：:Engineering strategies were applied to improve the CO(2) fixation rate and carbohydrate/lipid production of a Scenedesmus obliquus CNW-N isolate. The light intensity that promotes cell growth, carbohydrate/lipid productivity, and CO(2) fixation efficiency was identified. Nitrogen starvation was also employed to trigge...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.11.133
更新日期：2012-06-01 00:00:00
abstract：:Oil palm empty fruit bunch (OPEFB), contains abundant cellulose and hemicelluloses and can be used as a renewable resource for fuel and chemical production. This study, as the first attempt, aims to convert OPEFB derived sugars to polyhydroxybutyrate (PHB). OPEFB collected from a Malaysia palm oil refinery plant was c...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.08.029
更新日期：2013-11-01 00:00:00
abstract：:The ability of coagulation active proteins from common bean (Phaseolus vulgaris) seed for the removal of water turbidity was studied. Partial purification of protein coagulant was performed by precipitation with ammonium sulphate, dialysis and anion exchange chromatography. Adsorption parameters for ion-exchange proce...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.11.020
更新日期：2010-04-01 00:00:00
abstract：:When Chlorella biomass was hydrolyzed in the presence of 2% HCl and 2.5% MgCl(2), a sugar concentration of nearly 12%, and a sugar recovery of about 83% was obtained. Fermentation experiments demonstrated that glucose in the Chlorella biomass hydrolysates was converted into ethanol by Saccharomyces cerevisiae with a y...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.08.051
更新日期：2011-11-01 00:00:00
abstract：:An integrated ethanol-methane fermentation system was proposed to resolve wastewater pollution in cassava ethanol production. In the integrated system, wastewater originating from ethanol distillation was treated by two-stage anaerobic digestion and then used in medium for the next batch of ethanol fermentation. Ammon...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.06.032
更新日期：2012-09-01 00:00:00
abstract：:1,3-Propanediol (1,3-PDO) production from crude glycerol, a byproduct from biodiesel manufacturing, by Clostridium beijerinckii DSM 791 was studied with corn steep liquor as an inexpensive nitrogen source replacing yeast extract in the fermentation medium. A stable, long-term 1,3-PDO production from glycerol was demon...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.04.020
更新日期：2016-07-01 00:00:00
abstract：:Pharmaceuticals in surface waters and wastewater treatment plants (WWTPs) as emerging pollutants have become a major concern. In comparison with other wastewater treatments, removal of pharmaceuticals in MBR has received much attention. This review presents the source and occurrence of pharmaceuticals in WWTPs influen...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2018.08.029
更新日期：2018-11-01 00:00:00
abstract：:The main objective of this paper is to find the optimum operating condition to upgrade the EFB-derived pyrolysis oil (bio-oil) to liquid fuel, mainly gasoline using Taguchi Method. From the analysis that has been done, it is found that the optimum operating condition for heterogeneous catalytic cracking process is at ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.05.036
更新日期：2010-11-01 00:00:00
abstract：:The aim of this work deals with the development of new approaches to the production of furfural from xylose. It combines relatively cheap heterogeneous catalysts (Amberlyst 70) with simultaneous furfural stripping using nitrogen under semi-batch conditions. Nitrogen, compared to steam, does not dilute the vapor phase ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.05.015
更新日期：2011-08-01 00:00:00
abstract：:White-rot fungus Phlebia sp. MG-60 was identified as a good producer of ethanol from several cellulosic materials containing lignin. When this fungus was cultured with 20 g/L unbleached hardwood kraft pulp (UHKP), 8.4 g/L ethanol was produced after 168 h of incubation giving yields of ethanol of 0.42 g/g UHKP, 71.8% o...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.02.109
更新日期：2012-05-01 00:00:00
abstract：:An aerobic denitrifier was newly isolated and identified as Pseudomonas stutzeri strain XL-2. Strain XL-2 removed 97.9% of nitrate with an initial concentration about 100 mg/L. Nitrogen balance indicates that 12.4% of the initial nitrogen was converted to N2O, and 62.4% was converted to N2. Single factor experiments i...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.11.038
更新日期：2018-02-01 00:00:00
abstract：:Although the combination of endogenous partial denitrification (EPD) and Anammox (EPD-AMX) were developed for deep-level nitrogen removal, the effects of different carbon source were not clear. In this study, the EPD performance was investigated comparatively with acetate (EPDA) and glucose (EPDG). Results revealed th...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.124405
更新日期：2021-01-01 00:00:00
abstract：:(2S,3S)-2,3-Butanediol ((2S,3S)-2,3-BD) is a crucial chiral compound that acts as an excellent building block in asymmetric synthesis of highly valuable chiral compounds. However, the low concentration and optical purity of (2S,3S)-2,3-BD produced in previous studies limited its applications. In the present work, the ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.08.097
更新日期：2012-07-01 00:00:00
abstract：:Photoautotrophic fatty acid production of a highly CO(2)-tolerant green alga Chlorococcum littorale was investigated in the presence of inorganic carbon and nitrate at 295 K and a light intensity of 170 micromol-photon m(-2) s(-1). CO(2) concentration in the bubbling gas was adjusted by mixing pure gas components of C...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.05.048
更新日期：2009-11-01 00:00:00
abstract：:A process for the fermentative production of propionic acid from whole wheat flour using starch and gluten as nutrients is presented. Hydrolysis of wheat flour starch using amylases was optimized. A batch fermentation of hydrolysate supplemented with various nitrogen sources using Propionibacterium acidipropionici NRR...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.12.154
更新日期：2013-02-01 00:00:00
abstract：:The main objective of the present study is aimed to optimize the process parameters for the production of glucose from karanja seed cake. The Taguchi robust design method with L9 orthogonal array was applied to optimize hydrolysis reaction conditions and maximize sugar yield. Effect of temperature, acid concentration,...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.05.065
更新日期：2014-08-01 00:00:00
abstract：:A genetic algorithm has been used to optimize the composition of the culture medium for growing the microalga Nannochloropsis gaditana, based on the nutrients composition of the commercial medium ALGAL. This strategy was carried out through the implementation of 270 experiments spread over nine generations, which allo...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.11.057
更新日期：2015-02-01 00:00:00
abstract：:A field experiment was conducted on a deep Vertisol of Bhopal, India to compare root and shoot biomass, chlorophyll content, enzyme activity and nodulation in three cropping systems at three combinations of organic manure and inorganic-fertilizer: 75%NPK + 5 t farmyard manure (FYM), 75%NPK + 1.5 t poultry manure (PM),...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2004.02.012
更新日期：2004-10-01 00:00:00
abstract：:This project analyses the biogas potential of the halophyte Chenopodium quinoa Willd. In a first approach C. quinoa was grown with different concentrations of NaCl (0, 10 and 20 ppt NaCl) and the crop residues were used as substrate for biogas production. In a second approach, C. quinoa was grown with 0, 10, 20 and 30...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.12.061
更新日期：2016-03-01 00:00:00
abstract：:Inulin consists of linear chains of β-2,1-linked D-fructofuranose molecules terminated by a glucose residue through a sucrose-type linkage at the reducing end. In this review article, inulin and its applications in bioprocesses are overviewed. The tubers of many plants, such as Jerusalem artichoke, chicory, dahlia, an...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2010.12.086
更新日期：2011-03-01 00:00:00
abstract：:In the present work, we introduced a novel approach for microbial fatty acids (FA) production. Photosynthetic bacteria, Rhodobacter sphaeroides KD131, were cultivated in a continuous-flow, stirred-tank reactor (CFSTR) at various substrate (lactate) concentrations. At hydraulic retention time (HRT) 4d, cell concentrati...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.08.078
更新日期：2013-11-01 00:00:00
abstract：:This project aimed at producing a concentrated nitrogen fertilizer from liquid swine manure using electrodialysis (ED) and reverse osmosis (RO), as a mean to help resolve the excess nutrient problem faced by many swine producers, and offer an alternative to chemical nitrogen fertilizer production. Different types of E...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2006.12.039
更新日期：2008-10-01 00:00:00
abstract：:We assessed the suitability of various biogenic materials for development of a gradient packed bed bio-filter to mitigate the methane (CH4) emission from landfills. Five different biogenic materials (windrow compost-WC; vermicompost-VC; landfill top cover-LTC; landfill bottom soil-LBS; and river soil sediment-SS) were...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.01.059
更新日期：2016-10-01 00:00:00
abstract：:Fed-batch culture and the transformation conditions of Haematococcus pluvialis in a 5L photobioreactor were investigated. Methods of feeding model, low temperature at night and proper feeding time were used to increase both cell biomass and cell activity. Dry cell weight of 1.87 g/L which was 2.0-fold of batch culture...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.02.101
更新日期：2015-06-01 00:00:00
abstract：:A controlled and up-scalable route for the biosynthesis of silver nanopartilces (NPs) mediated by fungal proteins of Coriolus versicolor has been undertaken for the first time. The fungus when challenged with silver nitrate solution accumulated silver NPs on its surface in 72h which could be reduced to 1h by tailoring...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2008.05.048
更新日期：2009-01-01 00:00:00
abstract：:Microbial production of fuel and chemical feedstock is a promising approach to solving energy and environmental problems. n-Butanol, isobutanol and other higher alcohols are of particular interest because they can serve as both fuel and chemical feedstock. Alternative resources such as CO2, syngas, waste protein, and ...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2012.09.104
更新日期：2013-05-01 00:00:00
abstract：:Statistical optimization designs were used to optimize the phenol degradation using Chlorella pyrenoidosa. The important factor influencing phenol degradation was identified by two-level Plackett-Burman Design (PBD) with five factors. PBD determined the following three factors as significant for phenol degradation viz...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.01.138
更新日期：2016-05-01 00:00:00
abstract：:A pilot-scale chemical-biological process was performed efficiently to remove corrosive H2S from biogas. Consecutive 356-d livestock biogas purification was conducted at various H2S loading rates. The average inlet H2S concentration was 3542 ppm, and a removal efficiency of 95% was achieved with gas retention time of ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.10.040
更新日期：2013-05-01 00:00:00
