epsilon-Poly-L-lysine production by immobilized cells of Kitasatospora sp. MY 5-36 in repeated fed-batch cultures.

Abstract:

:The production of epsilon-PL by Kitasatospora sp. MY 5-36 through entrapment or adsorption on bagasse, synthetic sponge, macroporous silica gel, and loofah sponge was investigated in shake flask cultures, and immobilization on loofah sponge gave the highest epsilon-PL production. Repeated fed-batch cultures for epsilon-PL production were also carried out in a stirred bioreactor and final epsilon-PL concentrations and productivity of 34.11 g L(-1) and 9.34 g L(-1)d(-1), respectively were achieved by cells immobilize in loofah sponge. These values exceeded those for cultures with free cells (22.53 g L(-1) and 3.30 g L(-1)d(-1)). The immobilized cells were reused five times over a period of 526 h. These results suggest that the immobilization approach is promising for industrial applications.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Zhang Y,Feng X,Xu H,Yao Z,Ouyang P

doi

10.1016/j.biortech.2010.02.021

subject

Has Abstract

pub_date

2010-07-01 00:00:00

pages

5523-7

issue

14

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(10)00292-0

journal_volume

101

pub_type

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