Abstract:
:In this study, a novel indigo-producing bacterial strain PP-2 was isolated from activated sludge. It was identified as Acinetobacter sp. according to phylogenetic similarity of 16S rRNA gene sequence. This isolate was able to produce indigo from indole by utilizing a wide range of aromatic hydrocarbons. The results of SDS-PAGE analysis showed that the enzyme system induced by phenol was more abundant than that induced by other aromatic hydrocarbons. And the effects of metal ions on indigo production were also investigated, which indicated that the activity of cells induced with phenol could be inhibited by 0.5mM Fe(3+). Response surface methodology (RSM) was applied to optimize the process of indigo bio-production. The results exhibited that the maximal yield was achieved with 157.92 mg L(-1) phenol and 205.32 mg L(-1) indole. Under the optimal conditions, the indigo yield and transformation efficiency of indole were 202.92 mg L(-1) and 88%, respectively.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Qu Y,Pi W,Ma F,Zhou J,Zhang Xdoi
10.1016/j.biortech.2010.01.033subject
Has Abstractpub_date
2010-06-01 00:00:00pages
4527-32issue
12eissn
0960-8524issn
1873-2976pii
S0960-8524(10)00090-8journal_volume
101pub_type
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