Abstract：
:Linked to the development of cellulolytic enzyme cocktails from Myceliophthora thermophila, we studied the effect of different additives on the enzymatic hydrolysis yield. The hydrolysis of pretreated corn stover (PCS), sugar cane straw (PSCS) and microcrystalline cellulose (Avicel) was performed under industrial conditions using high solid loadings, limited mixing, and low enzyme dosages. The addition of polyethylene glycol (PEG4000) allowed to increase the glucose yields by 10%, 7.5%, and 32%, respectively in the three materials. PEG4000 did not have significant effect on the stability of the main individual enzymes but increased beta-glucosidase and endoglucanase activity by 20% and 60% respectively. Moreover, the presence of PEG4000 accelerated cellulase-catalyzed hydrolysis reducing up to 25% the liquefaction time. However, a preliminary economical assessment concludes that even with these improvements, a lower contribution of PEG4000 to the 2G bioethanol production costs would be needed to reach commercial feasibility.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Rocha-Martín J,Martinez-Bernal C,Pérez-Cobas Y,Reyes-Sosa FM,García BDdoi
10.1016/j.biortech.2017.06.132subject
Has Abstractpub_date
2017-11-01 00:00:00pages
48-56issue
Pt 1eissn
0960-8524issn
1873-2976pii
S0960-8524(17)31036-2journal_volume
244pub_type
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