Stimulation of the biosynthesis of carotenes by oxidative stress in Blakeslea trispora induced by elevated dissolved oxygen levels in the culture medium.

Abstract:

:The adaptive response of the fungus Blakeslea trispora to the oxidative stress induced by elevated dissolved oxygen concentrations during carotene production was investigated by measuring the specific activities of catalase (CAT) and superoxide dismutase (SOD) and the micromorphology of the fungus using a computerized image analysis system. Changes in the ratio of the volume of air (V(a)) over the medium and the volume of medium (V(m)) in the flask caused changes of the morphology of microorganism from clumps to pellets and increases in the specific activities of CAT and SOD. The oxidative stress in B. trispora resulted in a significant increase in carotene production, and a maximum proportion of β-carotene (60%), γ-carotene (50%), and lycopene (10%) (as percentages of total carotenes) was observed at a ratio V(a)/V(m) of 15.7, 4.0 and 1.5, respectively. The highest concentration of carotenes (115.0mg/g dry biomass) was obtained in V(a)/V(m) ratio of 9.0.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Nanou K,Roukas T

doi

10.1016/j.biortech.2011.06.027

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

8159-64

issue

17

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(11)00830-3

journal_volume

102

pub_type

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