Abstract：
:The development of a cost-effective hydrolysis for crude cellulose is an essential part of biorefinery developments. To establish such high solid hydrolysis, a new solid state reactor with static mixing is used. However, concentrations >10% (w/w) cause a rate and yield reduction of enzymatic hydrolysis. By optimizing the synergetic activity of cellulolytic enzymes at solid concentrations of 9%, 17% and 23% (w/w) of crude Organosolv cellulose, glucose concentrations of 57, 113 and 152 g L(-1) are reached. However, the glucose yield decreases from 0.81 to 0.72 g g(-1) at 17% (w/w). Optimal conditions for hydrolysis scale-up under minimal enzyme addition are identified. As result, at 23% (w/w) crude cellulose the glucose yield increases from 0.29 to 0.49 g g(-1). As proof of its applicability, biobutanol, succinic and itaconic acid are produced with the crude hydrolysate. The potential of the substrate is proven e.g. by a high butanol yield of 0.33 g g(-1).
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Tippkötter N,Duwe AM,Wiesen S,Sieker T,Ulber Rdoi
10.1016/j.biortech.2014.06.052subject
Has Abstractpub_date
2014-09-01 00:00:00pages
447-55eissn
0960-8524issn
1873-2976pii
S0960-8524(14)00896-7journal_volume
167pub_type
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