Abstract:
:The aim of this study was the production of amylolytic enzymes by solid state or submerged fermentations (SSF or SF, respectively), followed by purification using chemical process or microfiltration and immobilization of purified enzymes in a polyurethane support. The free and immobilized enzymes obtained were used to evaluate enzymatic hydrolysis of the polysaccharides of Spirulina. Microfiltration of the crude extracts resulted in an increase in their specific activity and thermal stability at 40°C and 50°C for 24h, as compared to extracts obtained by SSF and SF. Immobilization of polyurethane purified enzyme produced yields of 332% and 205% for the enzymes obtained by SF and SSF, respectively. Free or immobilized enzymes favor the generation of fermentable sugar, being the application of the purified and immobilized enzymes in the hydrolysis of microalgal polysaccharides considered a promising alternative towards development of the bioethanol production process from microalgal biomass.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Rodrigues ÉF,Ficanha AMM,Dallago RM,Treichel H,Reinehr CO,Machado TP,Nunes GB,Colla LMdoi
10.1016/j.biortech.2016.11.047subject
Has Abstractpub_date
2017-02-01 00:00:00pages
134-141eissn
0960-8524issn
1873-2976pii
S0960-8524(16)31562-0journal_volume
225pub_type
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