Optimization of recombinant hyperthermophilic esterase production from agricultural waste using response surface methodology.

Abstract:

:The aim of this work was to evaluate the capability of corn steep liquor being a low cost substrate on the recombinant protein production by cultivating recombinant Escherichia coli and increasing the production of hyperthermophilic esterase (HE). The effect of corn steep liquor, mineral salt and trace metals on hyperthermophilic esterase production was investigated by means of a five-level three-factor central composite rotatable design. Optimized values of the factors were determined and a maximum hyperthermophilic esterase production of 251.39 U/ml was obtained. This value equaled the yield by yeast extract and peptone medium on the whole.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Ren X,Yu D,Han S,Feng Y

doi

10.1016/j.biortech.2005.10.027

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

2345-9

issue

18

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(05)00507-9

journal_volume

97

pub_type

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