Abstract：
:The on-site production of cellulases is an important strategy for the development of sustainable second-generation ethanol production processes. This study concerns the use of a specific cellulolytic enzyme complex for hydrolysis of pretreated sugar cane bagasse. Glycosyl hydrolases (FPase, xylanase, and β-glucosidase) were produced using a new strain of Trichoderma harzianum, isolated from the Amazon rainforest and cultivated under different conditions. The influence of the carbon source was first investigated using shake-flask cultures. Selected carbon sources were then further studied under different pH conditions using a stirred tank bioreactor. Enzymatic activities up to 121 FPU/g, 8000 IU/g, and 1730 IU/g of delignified steam-exploded bagasse+sucrose were achieved for cellulase, xylanase and β-glucosidase, respectively. This enzymatic complex was used to hydrolyze pretreated sugar cane bagasse. A comparative evaluation, using an enzymatic extract from Trichoderma reesei RUTC30, indicated similar performance of the T. harzianum enzyme complex, being a potential candidate for on-site production of enzymes.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Delabona Pda S,Farinas CS,da Silva MR,Azzoni SF,Pradella JGdoi
10.1016/j.biortech.2011.12.048subject
Has Abstractpub_date
2012-03-01 00:00:00pages
517-21eissn
0960-8524issn
1873-2976pii
S0960-8524(11)01794-9journal_volume
107pub_type
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