Immobilization of laccase onto spacer-arm attached non-porous poly(GMA/EGDMA) beads: application for textile dye degradation.

Abstract:

:Non-porous poly(glycidyl methacrylate/ethyleneglycol dimetacrylate) (poly(GMA/EGDMA)) beads were prepared by suspension polymerization. The enzyme (i.e. laccase) was covalently immobilized onto plain and spacer-arm attached poly(GMA/EGDMA) beads. The amount of immobilized enzyme on the plain and spacer-arm attached beads was determined as 5.6 and 4.9 mg/g, respectively. The maximum activity (V(max)) and Michaelis constant (K(m)) of laccase immobilized on the spacer-arm attached beads, were found to be 77.6 U/min and 0.47 mM, respectively. Finally, the immobilized laccase was operated in a batch system, and textile dye Reactive Red 120 was successfully decolorized in the enzyme reactor.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Arica MY,Altintas B,Bayramoğlu G

doi

10.1016/j.biortech.2008.07.038

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

665-9

issue

2

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(08)00655-X

journal_volume

100

pub_type

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