Abstract：
:Ethanol production by a recombinant bacterium from wheat straw (WS) at high solid loading by separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) was studied. The yield of total sugars from dilute acid pretreated WS (150 g/L) after enzymatic saccharification was 86.3±1.5 g/L. The pretreated WS was bio-abated by growing a fungal strain aerobically in the liquid portion for 16 h. The recombinant Escherichia coli strain FBR5 produced 41.1±1.1 gethanol/L from non-abated WS hydrolyzate (total sugars, 86.6±0.3 g/L) in 168 h at pH7.0 and 35 °C. The bacterium produced 41.8±0.0 g ethanol/L in 120 h from the bioabated WS by SHF. It produced 41.6±0.7 g ethanol/L in 120 h from bioabated WS by fed-batch SSF. This is the first report of the production of above 4% ethanol from a lignocellulosic hydrolyzate by the recombinant bacterium.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Saha BC,Nichols NN,Cotta MAdoi
10.1016/j.biortech.2011.09.041subject
Has Abstractpub_date
2011-12-01 00:00:00pages
10892-7issue
23eissn
0960-8524issn
1873-2976pii
S0960-8524(11)01327-7journal_volume
102pub_type
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