Effect of biomass concentration and inoculum source on the rate of anaerobic cellulose solubilization.

Abstract:

:This study determines cellulose solubilization kinetics from controlled batch digestions and shows the effect of inoculum biomass concentrations. Separate measurements and analyzes were performed for sessile biomass (biofilms) and planktonic biomass (free suspensions). Experiments were conducted using either leachate enriched on cellulose or rumen fluid as inoculum to assess if the effect of biomass concentration was consistent for microbial populations from different source environments. All batch digestions were fitted to a first-order kinetic model (R(2) ranging from 0.94 to 0.99). Regression analysis used to compare the first-order hydrolysis rate showed that the first-order hydrolysis rate was most strongly correlated with the concentration of sessile biomass rather than with the concentration of total or planktonic biomass. The correlation between solubilization rate and sessile biomass was statistically the same for the rumen and leachate inoculated reactors indicating that at low concentration ratios of inoculum to cellulose, the rate of cellulose solubilization is dependant primarily on sessile biomass concentration rather than the species profile of the cellulolytic community.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Jensen PD,Hardin MT,Clarke WP

doi

10.1016/j.biortech.2009.05.018

subject

Has Abstract

pub_date

2009-11-01 00:00:00

pages

5219-25

issue

21

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(09)00558-6

journal_volume

100

pub_type

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