Intracellular storage of acetate/starch mixture by fast growing microbial culture in sequencing batch reactor under continuous feeding.

Abstract:

:The paper evaluated intracellular storage formation in fast growing microbial culture fed with acetate/starch mixture under continuous feeding. Three parallel laboratory-scale sequencing batch reactors (SBRs) were operated at a sludge age of 2 days: one of the SBRs was fed with acetate/starch mixture and the other two with acetate and starch, respectively, for comparing the results with single substrate systems. Despite continuous feeding, both acetate and starch components in the substrate mixture were partially converted to storage biopolymers. Poly-hydroxybutyrate (PHB) and glycogen pools were formed during SBR operation at steady state. Only a limited fraction of 12% of the acetate fed during each cycle generated PHB storage while the rest was directly utilized for microbial growth. Around half of the starch fraction of the substrate mixture was converted to glycogen. Increasing the sludge age to 8 days did not affect storage stoichiometry both for acetate and starch in the mixture.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Ciggin AS,Majone M,Orhon D

doi

10.1016/j.biortech.2012.06.003

subject

Has Abstract

pub_date

2012-09-01 00:00:00

pages

331-8

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(12)00906-6

journal_volume

119

pub_type

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