Abstract:
:A new sugar nucleotide cycling (SNC) process was established in a one-pot three enzyme-coupled reaction using disaccharide trehalose. Trehalose synthase from Pyrococcus horikoshii could be applied to the SNC process for the synthesis of functional α-galactosyl oligosaccharides, α-galactose (Gal) epitopes and globotriose, using the effective regeneration of UDP-Gal. The α-Gal epitopes and globotriose were found to attach to the cell-surface of enteropathogenic Escherichia coli O127 (EPEC) which were bound to human Caco-2 cells. These α-galactosyl oligosaccharides were able to prohibit the attachment of EPEC, which could have resulted in colonization and disease. The α-Gal epitope III with a lactulose acceptor showed the most inhibitory activity of anti-adhesion. The results suggest that the α-galactosyl oligosaccharides may be alternative anti-adhesion molecules that overcome antibiotic resistance.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Ryu SI,Woo JB,Lee SBdoi
10.1016/j.biortech.2013.03.049subject
Has Abstractpub_date
2013-05-01 00:00:00pages
743-6eissn
0960-8524issn
1873-2976pii
S0960-8524(13)00417-3journal_volume
136pub_type
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