Abstract：
:In order to develop feasible production processes for microalgal biodiesel, the isolation of high neutral lipid producing microalgae is crucial. Since the established Nile Red (NR) method for detection of intracellular lipids has been successful only for some microalgae, a more broadly applicable detection method would be desirable. Therefore, BODIPY 505/515, a lipophilic bright green fluorescent dye was tested for detection of intracellular lipids in Chlorella vulgaris, Dunaliella primolecta and Chaetoceros calcitrans. An optimum concentration of 0.067 μg ml(-1) was determined for lipid staining in the microalgae. Compared to NR, BODIPY 505/515 was more effective in staining microalgae and showed resistance to photobleaching, maintaining its fluorescence longer than 30 min.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Govender T,Ramanna L,Rawat I,Bux Fdoi
10.1016/j.biortech.2012.03.024subject
Has Abstractpub_date
2012-06-01 00:00:00pages
507-11eissn
0960-8524issn
1873-2976pii
S0960-8524(12)00442-7journal_volume
114pub_type
杂志文章abstract：:A lipase gene (Lip) of the Aspergillus niger was de novo synthesized and expressed in the Trichoderma reesei under the promoter of the cellobiohydrolase I gene (cbh1). RNAi-mediated gene silencing was successfully used to further improve the recombinant lipase production via down-regulation of CBHI which comprised mor...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.01.013
更新日期：2012-04-01 00:00:00
abstract：:Agave tequilana fructans (ATF) constitute a substrate for bioethanol and tequila industries. As Kluyveromyces marxianus produces specific fructanases for ATF hydrolysis, as well as ethanol, it can perform simultaneous saccharification and fermentation. In this work, fifteen K. marxianus yeasts were evaluated to develo...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.07.078
更新日期：2013-10-01 00:00:00
abstract：:Under certain conditions bacteria can act as a good biosorbent for different toxic heavy metals. However, no study on this aspect has been reported in case of acidophilic, heterotrophic, Gram-negative Acidiphilium strains, which are mostly resistant to several heavy metals. FTIR, SEM, TEM along with sorption experimen...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.12.100
更新日期：2012-03-01 00:00:00
abstract：:Microalgae are an attractive biofuel feedstock because of their high lipid to biomass ratios, lipid compositions that are suitable for biodiesel production, and the ability to grow on varied carbon sources. While algae can grow autotrophically, supplying an exogenous carbon source can increase growth rates and allow h...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.11.098
更新日期：2015-03-01 00:00:00
abstract：:In this study, a rhamnolipid biosurfactant-producing strain, Pseudomonas aeruginosa F-2, was used to recover oil from refinery oily sludge in laboratory and pilot-scale experiments. The optimum values of carbon to nitrogen ratio, temperature, sludge-water ratio and inoculum size for oil recovery were determined as 10,...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.04.024
更新日期：2012-07-01 00:00:00
abstract：:In this study, CRISPRi (clustered regularly interspaced short palindromic repeats interference) was used for the first time to regulate expression of exogenously supplied rfp gene as a proof-of-concept, and endogenous PEPC1 gene as a proof-of-function in Chlamydomonas reinhardtii. The efficiency of 94% and stability o...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.04.111
更新日期：2017-12-01 00:00:00
abstract：:A modified oxidation ditch (MOD) with an adjustable volume intraclarifier was proposed and used to achieve aerobic sludge granulation in continuous flow process. This MOD with working volume of 60L treated onsite wastewater from a town. Excellent aerobic granules with mean diameter of 600μm and sludge volume index (SV...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.01.130
更新日期：2014-04-01 00:00:00
abstract：:Polyhydroxyalkanoates (PHA) production from fermented thermal-hydrolyzed sludge was conducted by mixed microbial cultures (MMCs) in the study. An MMC enriched in the species Brachymonas_denitrificans (60.18%) was selected under an aerobic feast/famine regime, which is capable of denitrification and accumulating PHA. T...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.121895
更新日期：2019-11-01 00:00:00
abstract：:The hydrolysis rate coefficients of sorted municipal waste were evaluated from the biochemical methane potential tests using non-linear regression. A distributed mathematical model of anaerobic digestion of rich (food) and lean (non-food) solid wastes with greatly different rates of polymer hydrolysis/acidogenesis was...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2003.10.034
更新日期：2004-08-01 00:00:00
abstract：:Pseudomonas sp. C27 is a facultative autotrophic bacterium that can grow mixotrophically to undergo denitrifying sulfide removal (DSR) reactions with both organic matters and sulfide as electron donors. A detailed understanding of how the C27 strain simultaneously removes nitrogen, sulfur and carbon from water is crit...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.122169
更新日期：2019-12-01 00:00:00
abstract：:Three organosolv and three alkaline hemicellulosic fractions were prepared from lignocellulosic biomass of the fast-growing shrub Tamarix austromongolica (Tamarix Linn.). Sugar analysis revealed that the organosolv-soluble fractions contained a higher content of glucose (33.7-6.5%) and arabinose (14.8-5.6%), and a low...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.03.012
更新日期：2011-05-01 00:00:00
abstract：:Metal mobilization and immobilization catalyzed by microbial action are key processes in environmental biotechnology. Metal mobilization from ores, mining wastes, or solid residues can be used for recovering metals and/or remediating polluted environments; furthermore, immobilization reduces the migration of metals; c...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2019.02.028
更新日期：2019-05-01 00:00:00
abstract：:Butyrate fermentation by immobilized Clostridium tyrobutyricum was successfully carried out in a fibrous bed bioreactor using cane molasses. Batch fermentations were conducted to investigate the influence of pH on the metabolism of the strain, and the results showed that the fermentation gave a highest butyrate produc...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.02.032
更新日期：2009-07-01 00:00:00
abstract：:A life cycle approach was adopted for energy, green house gas (GHG) emissions and renewability assessment for production of 1ton of Jatropha biodiesel. Allocation and displacement approaches were applied for life cycle inventory, process energy and process GHG emission attribution to co-products. The results of proces...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.01.142
更新日期：2012-04-01 00:00:00
abstract：:Changes in the chemical characteristics and biological parameters of Eisenia fetida were assessed by VER using (CO+VE) rabbit manure (Vo) spiked with sewage sludge (SS) or vinasse (V). Seven mixtures were used: Vo, control; Vo+SS at 10%, 30%, and 50% (SS1, SS2, and SS3); Vo+V at 10%, 30%, and 50% (V1, V2, and V3). SS ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.03.029
更新日期：2013-06-01 00:00:00
abstract：:During the fermentation process, dissolved oxygen values and carbon-to-nitrogen ratios are critical factors influencing DHA productivity. This study employed an intermittent oxygen feeding method to maintain a 50% dissolved oxygen level and produced a dissolved oxygen fluctuation environment to facilitate both Auranti...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.07.068
更新日期：2012-11-01 00:00:00
abstract：:A series of supported Ni catalysts including Ni/MgO, Ni/γ-Al2O3, Ni/α-Al2O3, Ni/SiO2 and Ni/ZrO2 was tested in CO2 reforming of toluene as a model compound of tar from biomass gasification in a fluidized bed reactor, and characterized by the means of temperature programmed reduction with hydrogen (H2-TPR), XRD, TEM an...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.09.054
更新日期：2011-01-01 00:00:00
abstract：:Saccharum (Saccharum spontaneum L. ssp. aegyptiacum (Willd.) Hack.), is a rapidly growing, wide ranging high-yield perennial, suitable for second generation bioethanol production. This study evaluated oxalic acid as a pretreatment for bioconversion. Overall sugar yields, sugar degradation products, enzymatic glucan hy...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.02.036
更新日期：2010-07-01 00:00:00
abstract：:Preparation of activated carbon has been attempted using KOH as activating agent by microwave heating from biodiesel industry solid residue, oil palm empty fruit bunch (EFBAC). The significance of chemical impregnation ratio (IR), microwave power and activation time on the properties of activated carbon were investiga...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.09.116
更新日期：2012-01-01 00:00:00
abstract：:It has been well documented that the yeasts isolated from different marine environments are so versatile that they can produce various fine chemicals, enzymes, bioactive substances, single cell protein and nanoparticles. Many genes related to the biosynthesis and regulation of these functional biomolecules have been c...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2015.12.039
更新日期：2016-02-01 00:00:00
abstract：:Three models (blocking laws, combined and resistance-in-series) were applied to identify the prevailing fouling mechanisms in a submerged membrane in an up-flow anaerobic sludge blanket reactor treating municipal wastewater. Experimental runs were carried out at lab-scale with filtration periods of 4 and 10 min, follo...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.08.017
更新日期：2018-11-01 00:00:00
abstract：:Agricultural use of organic residues offers an attractive method for their safe disposal and a valuable source of organic amendments and nutrients. A field experiment was conducted to investigate the influences of 0, 25, 50 and 100 t/ha spent mushroom compost (SMC), forced aeration compost (FAC) and inorganic fertiliz...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.06.034
更新日期：2008-05-01 00:00:00
abstract：:The 2-keto-L-gulonic acid (2-KLG) is the direct precursor for industrial vitamin C production. The main biosynthetic method for 2-KLG production is the classical two-step fermentation route. However, disadvantages of this method are emerging, including high consumption of energy, difficulties in strain screening, comp...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.124069
更新日期：2020-12-01 00:00:00
abstract：:Linked to the development of cellulolytic enzyme cocktails from Myceliophthora thermophila, we studied the effect of different additives on the enzymatic hydrolysis yield. The hydrolysis of pretreated corn stover (PCS), sugar cane straw (PSCS) and microcrystalline cellulose (Avicel) was performed under industrial cond...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.06.132
更新日期：2017-11-01 00:00:00
abstract：:This study was conducted to investigate the long-term impact of influent phosphorus concentration on denitrifying phosphorus removal and N2O production during denitrifying phosphorous removal process. The results showed that, denitrifying phosphate accumulating organisms (DPAOs) could become dominant populations quick...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.12.016
更新日期：2015-03-01 00:00:00
abstract：:In this work, multiple reuses of Rhodococcus ruber TH3 free cells for the hydration of acrylonitrile to produce acrylamide in a membrane dispersion microreactor were carried out. Through using a centrifuge, the reactions reached 39.9, 39.5, 38.6 and 38.0wt% of the final acrylamide product concentration respectively wi...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.03.134
更新日期：2015-01-01 00:00:00
abstract：:In this study, cell walls of microalgae were hydrolyzed for the production of reducing sugar by cellulase, which was immobilized onto an electrospun polyacrylonitrile (PAN) nanofibrous membrane. Since the nitrile groups of the PAN membrane were activated by the amidination reaction and covalent binding to the amino gr...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.06.100
更新日期：2010-11-01 00:00:00
abstract：:Spent sulphite liquor (SSL) was used as carbon source for the production of succinic acid using immobilized cultures of Actinobacillus succinogenes and Basfia succiniciproducens on two different supports, delignified cellulosic material (DCM) and alginate beads. Fed-batch immobilized cultures with A. succinogenes in a...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.03.132
更新日期：2017-08-01 00:00:00
abstract：:This work describes the production of polyhydroxyalkanoates (PHAs) as a side stream process on a municipal waste water treatment plant (WWTP) at different operation conditions. Therefore various tests were conducted regarding a high PHA production and stable PHA composition. Influence of substrate concentration, tempe...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.06.037
更新日期：2014-09-01 00:00:00
abstract：:Untreated or improperly treated wastewater has often been cited as the primary contamination source of groundwater. The use of decentralized wastewater treatment systems has applicability around the world since it obviates the need for extensive infrastructure development and expenditures. The use of a submerged flow ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/s0960-8524(03)00029-4
更新日期：2003-08-01 00:00:00
