Abstract:
:In this work, multiple reuses of Rhodococcus ruber TH3 free cells for the hydration of acrylonitrile to produce acrylamide in a membrane dispersion microreactor were carried out. Through using a centrifuge, the reactions reached 39.9, 39.5, 38.6 and 38.0wt% of the final acrylamide product concentration respectively within 35min in a four cycle reuse of free cells. In contrast, using a stirring tank, free cells could only be used once with the same addition speed of acrylonitrile with a microreactor. Through observing the dissolution behavior of acrylonitrile microdroplets in a free cell solution using a coaxial microfluidic device and microscope, it was found that the acrylonitrile microdroplets with a diameter of 75μm were rarely observed within a length of 2cm channel within 10s, which illustrated that the microreactor can intensify the reaction rate to reduce the inhibition of acrylonitrile and acrylamide.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Li J,Liu J,Chen J,Wang Y,Luo G,Yu Hdoi
10.1016/j.biortech.2015.03.134subject
Has Abstractpub_date
2015-01-01 00:00:00pages
198-204eissn
0960-8524issn
1873-2976pii
S0960-8524(15)00466-6journal_volume
187pub_type
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