Multiple reuses of Rhodococcus ruber TH3 free cells to produce acrylamide in a membrane dispersion microreactor.

Abstract:

:In this work, multiple reuses of Rhodococcus ruber TH3 free cells for the hydration of acrylonitrile to produce acrylamide in a membrane dispersion microreactor were carried out. Through using a centrifuge, the reactions reached 39.9, 39.5, 38.6 and 38.0wt% of the final acrylamide product concentration respectively within 35min in a four cycle reuse of free cells. In contrast, using a stirring tank, free cells could only be used once with the same addition speed of acrylonitrile with a microreactor. Through observing the dissolution behavior of acrylonitrile microdroplets in a free cell solution using a coaxial microfluidic device and microscope, it was found that the acrylonitrile microdroplets with a diameter of 75μm were rarely observed within a length of 2cm channel within 10s, which illustrated that the microreactor can intensify the reaction rate to reduce the inhibition of acrylonitrile and acrylamide.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Li J,Liu J,Chen J,Wang Y,Luo G,Yu H

doi

10.1016/j.biortech.2015.03.134

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

198-204

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(15)00466-6

journal_volume

187

pub_type

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