Simultaneous saccharification and fermentation of Agave tequilana fructans by Kluyveromyces marxianus yeasts for bioethanol and tequila production.

Abstract:

:Agave tequilana fructans (ATF) constitute a substrate for bioethanol and tequila industries. As Kluyveromyces marxianus produces specific fructanases for ATF hydrolysis, as well as ethanol, it can perform simultaneous saccharification and fermentation. In this work, fifteen K. marxianus yeasts were evaluated to develop inoculums with fructanase activity on ATF. These inoculums were added to an ATF medium for simultaneous saccharification and fermentation. All the yeasts, showed exo-fructanhydrolase activity with different substrate specificities. The yeast with highest fructanase activity in the inoculums showed the lowest ethanol production level (20 g/l). Five K. marxianus strains were the most suitable for the simultaneous saccharification and fermentation of ATF. The volatile compounds composition was evaluated at the end of fermentation, and a high diversity was observed between yeasts, nevertheless all of them produced high levels of isobutyl alcohol. The simultaneous saccharification and fermentation of ATF with K. marxianus strains has potential for industrial application.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Flores JA,Gschaedler A,Amaya-Delgado L,Herrera-López EJ,Arellano M,Arrizon J

doi

10.1016/j.biortech.2013.07.078

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

267-273

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(13)01146-2

journal_volume

146

pub_type

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