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Production of (R)-3-hydroxybutyric acid by fermentation and bioconversion processes with Azohydromonas lata.

Abstract：

:Feasibility of producing (R)-3-hydroxybutyric acid ((R)-3-HB) using wild type Azohydromonas lata and its mutants (derived by UV mutation) was investigated. A. lata mutant (M5) produced 780 m g/l in the culture broth when sucrose was used as the carbon source. M5 was further studied in terms of its specificity with various bioconversion substrates for production of (R)-3-HB. (R)-3-HB concentration produced in the culture broth by M5 mutant was 2.7-fold higher than that of the wild type strain when sucrose (3% w/v) and (R,S)-1,3-butanediol (3% v/v) were used as carbon source and bioconversion substrate, respectively. Bioconversion of resting cells (M5) with glucose (1% v/w), ethylacetoacetate (2% v/v), and (R,S)-1,3-butanediol (3% v/v), resulted in (R)-3-HB concentrations of 6.5 g/l, 7.3g/l and 8.7 g/l, respectively.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Ugwu CU,Tokiwa Y,Ichiba T

doi

10.1016/j.biortech.2011.03.073

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

6766-8

issue

12

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(11)00430-5

journal_volume

102

pub_type

杂志文章

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