Isolation of free-living dinitrogen-fixing bacteria and their activity in compost containing de-inking paper sludge.

Abstract:

:Knowledge of the microbiology of dinitrogen (N2)-fixing bacteria in compost rich in de-inking paper sludge (DPS) is limited. Dinitrogen (N2)-fixing bacteria from DPS composts were isolated and studied for their N2-fixing activity in vitro and in vivo. Two Gram-negative N2-fixing isolates were identified as Pseudomonas. At 20 degrees C, both isolates revealed that N2-fixing activity was higher than that of three arctic Pseudomonas strains. Their N2-fixing activity was found to occur between 18 and 25 degrees C, a pattern that was similar to the reference isolate Azotobacter ATCC 7486. Composts successfully showed N2-fixing activity after carbohydrate amendments both with and without inoculation of a N2-fixing isolate. These results suggest that DPS composts support N2-fixing bacteria and that N2-fixing activity is dependent on a usable carbohydrate source.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Beauchamp CJ,Lévesque G,Prévost D,Chalifour FP

doi

10.1016/j.biortech.2005.04.041

subject

Has Abstract

pub_date

2006-05-01 00:00:00

pages

1002-11

issue

8

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(05)00249-X

journal_volume

97

pub_type

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