Abstract:
:The mannan endo-1,4-β-mannosidase gene man26A from Aspergillus niger CBS 513.88 was optimized according to the codon usage bias in Pichia pastoris and synthesized by splicing overlap extension PCR. It was successfully expressed in P. pastoris using constitutive expression vector pGAPzαA. The recombinant endo-beta-1,4-mannanase could work in an extremely board temperature range and over 30% relative activity were retained in the temperature range of 5-60°C. The optimal pH value and temperature for activity were 5.0 and 45°C, respectively. It was highly thermotolerant with a half-life time of 15min at 90°C. A novel fed-batch strategy was developed successfully for high cell-density fermentation and mannanase activity reached 5069U/mL after cultivation for 56h in 50L fermenter. The broad working temperature range, high thermotolerance and efficient expression made this enzyme possible to be applied in food, animal feed and the production of biofuels.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zhao W,Zheng J,Zhou HBdoi
10.1016/j.biortech.2011.04.070subject
Has Abstractpub_date
2011-08-01 00:00:00pages
7538-47issue
16eissn
0960-8524issn
1873-2976pii
S0960-8524(11)00585-2journal_volume
102pub_type
杂志文章abstract::The use of non-food feedstocks to produce renewable microbial resources can limit our dependence on fossil fuels and lower CO2 emissions. Since microalgae display a virtuous CO2 and O2 exchange with heterotrophs, the microalga Chlamydomonas reinhardtii was combined with the oleaginous yeast Lipomyces starkeyi, known f...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2018.11.063
更新日期:2019-02-01 00:00:00
abstract::This review addresses the use of biochar as a green and versatile catalyst support for emerging high-end applications beyond soil remediation, including chemical synthesis and biodiesel production from biomass, and pollutant degradation in the environment. Their catalytic performances are comparable or even superior t...
journal_title:Bioresource technology
pub_type: 杂志文章,评审
doi:10.1016/j.biortech.2017.06.163
更新日期:2017-12-01 00:00:00
abstract::A process of intermittently cyclic activated sludge-membrane bioreactor (ICAS-MBR) was developed to enhance the performance of biological phosphorus removal (EBPR), which was constructed under sequencing anoxic/anaerobic/aerobic condition. The performance between ICAS-MBR and conventional anoxic/aerobic-membrane biore...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2009.02.054
更新日期:2009-09-01 00:00:00
abstract::The structural evolution characteristics of maize stalk/char particles during pyrolysis were investigated. The char was prepared by pyrolyzing at temperatures ranging from 200 to 900 degrees C. Maize stalk and chars were characterized by thermogravimetric analysis, ultimate analysis (TGA), Fourier transform infrared s...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2009.05.009
更新日期:2009-10-01 00:00:00
abstract::Knowledge of the microbiology of dinitrogen (N2)-fixing bacteria in compost rich in de-inking paper sludge (DPS) is limited. Dinitrogen (N2)-fixing bacteria from DPS composts were isolated and studied for their N2-fixing activity in vitro and in vivo. Two Gram-negative N2-fixing isolates were identified as Pseudomonas...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2005.04.041
更新日期:2006-05-01 00:00:00
abstract::The solvolysis behavior and reaction kinetics of the three components of biomass (cellulose, hemicelluloses and lignin) liquefied in polyhydric alcohols (PEG 400 or glycerol) were investigated in this paper. Three stages were observed during the solvolysis process and the main degradation stage could be further divide...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2016.09.008
更新日期:2016-12-01 00:00:00
abstract::In a separator electrode assembly microbial fuel cell, oxygen crossover from the cathode inhibits current generation by exoelectrogenic bacteria, resulting in poor reactor startup and performance. To determine the best approach for improving startup performance, the effect of acclimation to a low set potential (-0.2V,...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2013.01.036
更新日期:2013-04-01 00:00:00
abstract::Intensive livestock production is connected with a number of environmental effects, including discharges to soils and surface waters and emissions to the atmosphere. In areas with a high livestock density the low availability of nearby arable land, together with the preferred use of chemical fertilizer by arable farme...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2009.03.003
更新日期:2009-11-01 00:00:00
abstract::A new agricultural antibiotic, 2-heptyl-5-hexylfuran-3-carboxylic acid (HHCA), was recently discovered, but its further application was limited due to its low production titer. In shake flask fermentation, the effect of initial K(L)a within the range of 2.12-18.87 h⁻¹ on HHCA production was investigated. The cell grow...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2011.07.011
更新日期:2011-10-01 00:00:00
abstract::One of the hurdles of renewable energy production from photosynthetic microorganisms is separating the biomass from water in cultures. Bioflocculation with filamentous fungus Aspergillus niger, an alternative low-cost method used for such separation, was studied with four cyanobacteria. Cocultures with Spirulina maxim...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2019.122167
更新日期:2019-12-01 00:00:00
abstract::The Scenedesmus obliquus FSP-3, a species with excellent potential for CO(2) capture and lipid production, was harvested using dispersed ozone flotation. While air aeration does not, ozone produces effective solid-liquid separation through flotation. Ozone dose applied for sufficient algal flotation is similar to thos...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2010.04.083
更新日期:2011-01-01 00:00:00
abstract::This work deals with the capture of phosphorus from aqueous solutions by biosorption onto zirconium loaded okara (ZLO). The batch-mode experiments were conducted to examine the effect of pH, biosorbent dose, initial phosphorus concentration, contact time, and temperature on the process. It was found that, the adsorpti...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2014.07.069
更新日期:2014-10-01 00:00:00
abstract::Pine trees comprise over 35% of the forests in Korea, since 1989, pine wilt disease introduced via the Japanese pine sawyer has been infecting many of these trees. As a renewable resource, pine can be converted to bio-oil, gas, and char through pyrolysis. In this study, the pyrolysis characteristics of pine trees were...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2010.07.094
更新日期:2010-12-01 00:00:00
abstract::A bench-scale constructed wetland combined with microbial electrochemical technology (MET-CW) was run for 400days with groundwater contaminated with benzene, methyl-tert-butyl ether (MTBE), and ammonium (NH4(+)). Four vertically stacked anode modules were embedded into a sand bed and connected with a stainless steel c...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2015.07.111
更新日期:2015-11-01 00:00:00
abstract::This study evaluated the potential of bioflocculant production from Aspergillus niger using palm oil mill effluent (POME) as carbon source. The bioflocculant named PM-5 produced by A. niger showed a good flocculating capability and flocculating rate of 76.8% to kaolin suspension could be achieved at 60 h of culture ti...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2014.08.038
更新日期:2014-11-01 00:00:00
abstract::The organic fraction of municipal solid waste (OFMSW) is an abundant and sustainable substrate for the anaerobic digestion (AD) process, yet ammonia released during OFMSW hydrolysis could result in suboptimal biogas production. Acclimatized ammonia tolerant microorganisms offer an efficient way to alleviate ammonia in...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2019.121376
更新日期:2019-08-01 00:00:00
abstract::A thermophilic strain Bacillus subtilis GXA-28 with capability of γ-PGA production was characterized, and its product was identified. The effect of temperatures on cell growth, γ-PGA yield and molecular weight were investigated. Results showed that γ-PGA yield reached 19.92g/L at 45°C with a high productivity of 0.91g...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2013.12.086
更新日期:2014-03-01 00:00:00
abstract::This study prepared a carboxylic functionalized bioadsorbent that met the "4-E" criteria: Efficient, Economical, Environmentally friendly, and Easily-produced. Sesame straw (Sesamum indicum L.) was functionalized through treatment with citric acid (SSCA) and tartaric acid (SSTA). The products were examined for adsorpt...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2017.04.066
更新日期:2017-08-01 00:00:00
abstract::A combined process was designed for the co-production of ethanol and methane from unwashed steam-exploded corn stover. A terminal ethanol titer of 69.8 g/kg mass weight (72.5%) was achieved when the fed-batch mode was performed at a final solids loading of 35.5% (w/w) dry matter (DM) content. The whole stillage from h...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2014.05.044
更新日期:2014-08-01 00:00:00
abstract::This review describes the recent results in hydrothermal liquefaction (HTL) of biomass in continuous-flow processing systems. Although much has been published about batch reactor tests of biomass HTL, there is only limited information yet available on continuous-flow tests, which can provide a more reasonable basis fo...
journal_title:Bioresource technology
pub_type: 杂志文章,评审
doi:10.1016/j.biortech.2014.09.132
更新日期:2015-02-01 00:00:00
abstract::The aim of this study was to investigate the salinity uptake by natural zeolite when used as an ingredient during the composting process. The amounts of 5% and 10% of natural zeolite were applied to poultry litter as volume and compared with the compost made with no amendment. The results clearly showed that the salin...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2007.11.061
更新日期:2008-05-01 00:00:00
abstract::Viscosity trends in pretreated corn stover slurries undergoing enzymatic saccharification were characterized for a range of initial insoluble solids concentrations from 10% to 25% and correlated with total glucose released and changes in insoluble solids concentration throughout a 7day period. Viscosity trends are def...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2009.12.071
更新日期:2010-05-01 00:00:00
abstract::The coupling between a microalgal pond and an anaerobic digester is a promising alternative for sustainable energy production by transforming carbon dioxide into methane using solar energy. In this paper, we demonstrate the ability of the original ADM1 model and a modified version (based on Contois kinetics for the hy...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2011.04.015
更新日期:2011-07-01 00:00:00
abstract::Flax and sisal pulps were treated with two laccases (from Pycnoporus cinnabarinus, PcL and Trametes villosa, TvL, respectively), in the presence of different phenolic compounds (syringaldehyde, acetosyringone and p-coumaric acid in the case of flax pulp, and coniferaldehyde, sinapaldehyde, ferulic acid and sinapic aci...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2010.05.080
更新日期:2010-11-01 00:00:00
abstract::Methane production by anaerobic digestion (AD) of macroalgae (seaweed) is a promising algal bioenergy option. Work presented here is primarily based on the AD of Laminaria hyperborea using batch and continuously stirred tank reactors. Extrapolation of data from batch studies to long term continuous reactors was unreli...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2013.05.124
更新日期:2013-09-01 00:00:00
abstract::Investigating the release characteristics of alkali and alkaline earth metallic species (AAEMs) is of potential interest because of AAEM's possible useful service as catalysts in biomass thermal conversion. In this study, three kinds of typical Chinese biomass were selected to pyrolyse and their chars were subsequentl...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.03.051
更新日期:2012-07-01 00:00:00
abstract::Enzymatically synthesized lauroyl glucose emulsified different hydrophobic substrates when assayed spectrophotometrically. Stable emulsions were formed with triglycerides as well as with hydrocarbons. There was a linear relation between the concentration of lauroyl glucose (50-450 microg) and emulsification activity u...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2006.05.031
更新日期:2007-05-01 00:00:00
abstract::The cell growth rate and cellular lipid content of microalgae are affected by the nitrogen levels during cultivation. The growth rate and lipid content of marine microalga Tetraselmis sp. was found to increase under nitrate replete conditions, but not under deplete conditions. Thus, in order to enhance the lipid produ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2016.01.045
更新日期:2016-04-01 00:00:00
abstract::The use of algae for biofuel production is expected to play an important role in securing energy supply in the next decades. A consequential life cycle assessment (LCA) and an energy analysis of seaweed-based biofuel production were carried out in Nordic conditions to document and improve the sustainability of the pro...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.11.029
更新日期:2013-02-01 00:00:00
abstract::A N-doped couping MgO-modified biochar (MgO-N-BC) was synthesized from corncob-to-xylose residue by two-step slow pyrolysis method. The biochar exhibited a remarkable Pb(II) sorption capacity (maximum 1429 mg·g-1) in aqueous solution. The sorption of Pb(II) onto MgO-N-BC best fitted Freundlich model and pseudo-second-...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2020.123708
更新日期:2020-10-01 00:00:00