Abstract：
:A thermophilic strain Bacillus subtilis GXA-28 with capability of γ-PGA production was characterized, and its product was identified. The effect of temperatures on cell growth, γ-PGA yield and molecular weight were investigated. Results showed that γ-PGA yield reached 19.92g/L at 45°C with a high productivity of 0.91g/L/h, and the molecular weight reached 3.03×10(6)Da. Then, the flux distribution and the key enzyme activities at 2-oxoglutarate branch under specified temperature were determined to illustrate the possible metabolic mechanism contributing to the improved γ-PGA production. Results indicated that the fluxes from iso-citrate to 2-oxoglutarate and from 2-oxoglutarate to glutamate were increased with high activity of isocitrate dehydrogenase and glutamate dehydrogenase, which led to enhance γ-PGA production. This work firstly employed temperature control strategy to improve γ-PGA production, and provided novel information on the metabolic mechanism of γ-PGA biosynthesis in Bacillus species.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zeng W,Chen G,Wang Q,Zheng S,Shu L,Liang Zdoi
10.1016/j.biortech.2013.12.086subject
Has Abstractpub_date
2014-03-01 00:00:00pages
104-10eissn
0960-8524issn
1873-2976pii
S0960-8524(13)01926-3journal_volume
155pub_type
杂志文章abstract：:As antibiotic and heavy metals are over used in the livestock industry, animal manure is a reservoir of antibiotic resistance genes (ARGs). Anaerobic digestion has been reported to have the potential to reduce ARGs. However, few studies investigated whether reduction of ARGs would be affected by different external pre...
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