Abstract:
:In this study a complementary modeling and experimental approach was used to explore how growth controls the NADPH generation and availability, and the resulting impact on PHB (polyhydroxybutyrate) yields and kinetics. The results show that the anabolic demand allowed the NADPH production through the Entner-Doudoroff (ED) pathway, leading to a high maximal theoretical PHB production yield of 0.89 C mole C mole(-1); whereas without biomass production, NADPH regeneration is only possible via the isocitrate dehydrogenase leading to a theoretical yield of 0.67 C mole C mole(-1). Furthermore, the maximum specific rate of NADPH produced at maximal growth rate (to fulfil biomass requirement) was found to be the maximum set in every conditions, which by consequence determines the maximal PHB production rate. These results imply that sustaining a controlled residual growth improves the PHB specific production rate without altering production yield.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Grousseau E,Blanchet E,Déléris S,Albuquerque MG,Paul E,Uribelarrea JLdoi
10.1016/j.biortech.2013.08.120subject
Has Abstractpub_date
2013-11-01 00:00:00pages
30-8eissn
0960-8524issn
1873-2976pii
S0960-8524(13)01371-0journal_volume
148pub_type
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