Impact of sustaining a controlled residual growth on polyhydroxybutyrate yield and production kinetics in Cupriavidus necator.

Abstract:

:In this study a complementary modeling and experimental approach was used to explore how growth controls the NADPH generation and availability, and the resulting impact on PHB (polyhydroxybutyrate) yields and kinetics. The results show that the anabolic demand allowed the NADPH production through the Entner-Doudoroff (ED) pathway, leading to a high maximal theoretical PHB production yield of 0.89 C mole C mole(-1); whereas without biomass production, NADPH regeneration is only possible via the isocitrate dehydrogenase leading to a theoretical yield of 0.67 C mole C mole(-1). Furthermore, the maximum specific rate of NADPH produced at maximal growth rate (to fulfil biomass requirement) was found to be the maximum set in every conditions, which by consequence determines the maximal PHB production rate. These results imply that sustaining a controlled residual growth improves the PHB specific production rate without altering production yield.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Grousseau E,Blanchet E,Déléris S,Albuquerque MG,Paul E,Uribelarrea JL

doi

10.1016/j.biortech.2013.08.120

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

30-8

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(13)01371-0

journal_volume

148

pub_type

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