Abstract:
:Lipase-catalyzed production of palm esters by alcoholysis of palm oil with oleyl alcohol in n-hexane was performed in 2L stirred-tank reactor (STR). Investigation on the performance of reactor operation was carried out in batch mode STR with single impeller mounted on the centrally located shaft. Rushton turbine (RT) impellers provide the highest reaction yield (95.8%) at lower agitation speed as compared to AL-hydrofoil (AL-H) and 2-bladed elephant ear (EE) impellers. Homogenous enzyme particles suspension was obtained at 250 rpm by using RT impeller. At higher impeller speed, the shear effect on the enzyme particles caused by agitation has decreased the reaction performance. Palm esters reaction mixture in STR follows Newtons' law due to the linear relation between the shear stress (tau) and shear rate (dupsilon/dy). High stability of Lipozyme RM IM was observed as shown by its ability to be repeatedly used to give high percentage yield (79%) of palm esters even after 15 cycles of reaction. The process was successfully scale-up to 75 L STR (50 L working volume) based on a constant impeller tip speed approach, which gave the yield of 97.2% after 5h reaction time.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Keng PS,Basri M,Ariff AB,Abdul Rahman MB,Abdul Rahman RN,Salleh ABdoi
10.1016/j.biortech.2007.12.049subject
Has Abstractpub_date
2008-09-01 00:00:00pages
6097-104issue
14eissn
0960-8524issn
1873-2976pii
S0960-8524(07)01073-5journal_volume
99pub_type
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