Detection of algal lipid accumulation due to nitrogen limitation via dielectric spectroscopy of Chlamydomonas reinhardtii suspensions in a coaxial transmission line sample cell.

Abstract:

:In this study, dielectric characterization of algae cell suspensions was used to detect lipid accumulation due to nitrogen starvation. Wild-type Chlamydomonas reinhardtii (CC-125) was cultivated in replete and nitrogen-limited conditions in order to achieve a range of lipid contents, as confirmed by Nile Red fluorescence measurements. A vector network analyzer was used to measure the dielectric scattering parameters of a coaxial region of concentrated cell suspension. The critical frequency fc of the normalized transmission coefficient |S21(*)| decreased with increasing lipid content but did not change with cell concentration. These observations were consistent with a decrease in cytoplasmic conductivity due to lipid accumulation in the preliminary transmission line model. This dielectric sensitivity to lipid content will facilitate the development of a rapid, noninvasive method for algal lipid measurement that could be implemented in industrial settings without the need for specialized staff and analytical facilities.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Bono MS Jr,Ahner BA,Kirby BJ

doi

10.1016/j.biortech.2013.06.040

subject

Has Abstract

pub_date

2013-09-01 00:00:00

pages

623-31

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(13)00959-0

journal_volume

143

pub_type

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