Abstract:
:A process for the production of l-amino-acids concentrates from microalgae biomass by enzymatic hydrolysis has been developed. The process includes pre-treatment for cell-disruption, enzymatic hydrolysis and final separation by centrifugation. Thermal and mechanical cell-disruption methods have been tested, selecting mechanical disruption using bead milling for 30 min. The enzymatic hydrolysis was done using the commercial enzymes Alcalase and Flavourzyme. Maximum hydrolysis was obtained for biomass concentrations under 270 g/l and previous additional treatment with Viscozyme, reaching a 42% hydrolysis. Repeated reaction steps increased the hydrolysis from 42% (4h) with a single step to 59% (8h) after two successive steps. Further increase of the number of steps had a meagre impact on the global yield. The process widens the portfolio of products that can be obtained from microalgae biomass and is a new possibility to enhance the economic viability of microalgae-based biofuels production processes.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Romero García JM,Acién Fernández FG,Fernández Sevilla JMdoi
10.1016/j.biortech.2012.02.094subject
Has Abstractpub_date
2012-05-01 00:00:00pages
164-70eissn
0960-8524issn
1873-2976pii
S0960-8524(12)00345-8journal_volume
112pub_type
杂志文章abstract::Constructed wetland (CW) for wastewater treatment has attracted increasing attention. In this review, the system configuration optimization, purification effect and general mechanisms of nitrogen removal in CW are systematically summarized and discussed. Ammonia oxidation is a crucial and primary process for total nit...
journal_title:Bioresource technology
pub_type: 杂志文章,评审
doi:10.1016/j.biortech.2019.122086
更新日期:2019-12-01 00:00:00
abstract::A pot experiment was conducted in a greenhouse to evaluate the phytoremediation abilities of four forage grasses with respect to soil Cd and Zn pollution. High Cd pollution significantly increased the biomass of Pennisetum americanum (L.) LeekexPennisetum purpureum Schumach, showed no effect on Silphium perfoliatum Li...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2009.11.065
更新日期:2010-03-01 00:00:00
abstract::The on-site production of cellulases is an important strategy for the development of sustainable second-generation ethanol production processes. This study concerns the use of a specific cellulolytic enzyme complex for hydrolysis of pretreated sugar cane bagasse. Glycosyl hydrolases (FPase, xylanase, and β-glucosidase...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2011.12.048
更新日期:2012-03-01 00:00:00
abstract::This study investigated the feasibility of using recovered nutrients from hydrothermal carbonization (HTC) for cultivation of microalga Chlorella vulgaris. Different dilution multiples of 50, 100 and 200 were applied to the recycled process water from HTC and algal growth was compared among these media and a standard ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.09.062
更新日期:2012-12-01 00:00:00
abstract::During the fermentation process, dissolved oxygen values and carbon-to-nitrogen ratios are critical factors influencing DHA productivity. This study employed an intermittent oxygen feeding method to maintain a 50% dissolved oxygen level and produced a dissolved oxygen fluctuation environment to facilitate both Auranti...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.07.068
更新日期:2012-11-01 00:00:00
abstract::The biodegradation and mineralisation of hexadecane (HXD) by Aspergillus niger were studied in SmF and Solid-state fermentation (SSF). HXD concentrations ranging from 45 to 180 g/l (SSF) and from 20 to 80 g/l (SmF) were tested. HXD consumption was three times higher and fungal growth was up to 30 times faster in SSF t...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/s0960-8524(02)00207-9
更新日期:2003-03-01 00:00:00
abstract::The molecular structure of humic acid (HA) extracted was investigated by FT-IR and (13)C CP/MAS NMR spectroscopy during the vermicomposting of sugar industry wastes, viz. pressmud, trash and bagasse for 60days. A rapid decrease in C/N and lignocellulosic (lignin, cellulose, hemicellulose) content was observed in vermi...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2006.06.007
更新日期:2007-05-01 00:00:00
abstract::Current research aimed to increase mixotrophic biomass from various organic carbon sources by exploring best light conditions. Three substrates glucose, acetic acid and glycerol were studied for their effects on mixotrophic microalgae cultivation under four light conditions. Light irradiance exhibited variability in g...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2019.03.024
更新日期:2019-06-01 00:00:00
abstract::Photosynthetic bacteria have considerable biotechnological potential for biological hydrogen production due to higher substrate conversion efficiency and hydrogen yield. Phototrophic fermentation using photosynthetic bacteria has a major advantage of being able to further convert the byproducts originating from dark f...
journal_title:Bioresource technology
pub_type: 杂志文章,评审
doi:10.1016/j.biortech.2011.05.082
更新日期:2011-09-01 00:00:00
abstract::The use of different chemically modified cassava waste biomass for the enhancement of the adsorption of three metal ions Cd, Cu and Zn from aqueous solution is reported in this paper. Treating with different concentrations of thioglycollic acid modified the cassava waste biomass. The sorption rates of the three metals...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/s0960-8524(03)00145-7
更新日期:2003-12-01 00:00:00
abstract::Recently, vertical flow constructed wetlands (VFCWs) with intermittent aeration have been proven as an efficient technology to enhance removal efficiency of organics and nitrogen for wastewater treatment. However, the low denitrification effect in VFCWs was a problem for treating low carbon source wastewater. In this ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2017.05.072
更新日期:2017-10-01 00:00:00
abstract::In this work, the effect of incorporating an acidic ferrous sulphate waste (SF) over co-composting process of sewage sludge and olive mill solid wastes in a 1:2 v/v wet basis was investigated. The SF used was an industrial by-product of titanium oxide synthesis and its addition resulted in a chemical stabilisation of ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2007.12.013
更新日期:2008-09-01 00:00:00
abstract::Hydrothermal carbonization, an environmental friendly treatment method was employed to pretreat bamboo for hydrochar preparation in the present study. Hydrothermal carbonization could elevate the fuel properties and combustion behavior of bamboo. The combustion kinetic parameters of raw bamboo and hydrochars were calc...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2016.01.068
更新日期:2016-04-01 00:00:00
abstract::One of the key bottlenecks of the economically viable production of low added value microalgal products (food supplements, feed, biofuels) is the harvesting of cells from diluted culture medium. The main goals of this work were to prepare a novel flocculation agent based on spent brewer's yeast, a brewery by-product, ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2014.11.056
更新日期:2015-02-01 00:00:00
abstract::In the present investigation, recovery of malic acid from the fermentation broth was performed by using in situ reactive extraction method employing different combination of amine and solvent systems. Totally six solvent mixtures were tested for toxicity on Aspergillus niger PJR1. Further, effect of solvent mixture co...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2020.123259
更新日期:2020-07-01 00:00:00
abstract::The aim of this work was to study the biological catalysts and possible substrate conversion routes in mesophilic dark fermentation reactors aimed at producing H2 from olive mill wastewater. Bacillus and Clostridium were the most abundant phylotypes during the rapid stage of H2 production. Chemical analyses combined w...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2020.124157
更新日期:2021-01-01 00:00:00
abstract::Formic acid is the main breakdown product of mild hydrothermal treatment of carbohydrates. Further conversion to methanol was achieved using Cu as catalyst and Zn as reductant under hydrothermal conditions of 250-325 °C for 3-12 h. Both Cu and Zn used were commercial Cu and Zn powders with particle sizes of 200 mesh. ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.03.032
更新日期:2012-06-01 00:00:00
abstract::Indulin AT biodegradation by basidiomycetous fungi, actinobacteria and commercial laccases was evaluated using a suite of chemical analysis methods. The extent of microbial degradation was confirmed by novel thermal carbon analysis (TCA), as the treatments altered the carbon desorption and pyrolysis temperature profil...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2016.08.016
更新日期:2016-11-01 00:00:00
abstract::A pilot-scale chemical-biological process was performed efficiently to remove corrosive H2S from biogas. Consecutive 356-d livestock biogas purification was conducted at various H2S loading rates. The average inlet H2S concentration was 3542 ppm, and a removal efficiency of 95% was achieved with gas retention time of ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.10.040
更新日期:2013-05-01 00:00:00
abstract::Investigating the release characteristics of alkali and alkaline earth metallic species (AAEMs) is of potential interest because of AAEM's possible useful service as catalysts in biomass thermal conversion. In this study, three kinds of typical Chinese biomass were selected to pyrolyse and their chars were subsequentl...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.03.051
更新日期:2012-07-01 00:00:00
abstract::In solid-state anaerobic digestion (SS-AD) of cellulosic biomass, the volumetric methane production rate has often been found to increase with the increase in total solids (TS) content until a threshold is reached, and then to decrease. This phenomenon cannot be explained by conventional understanding derived from liq...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2014.05.114
更新日期:2014-09-01 00:00:00
abstract::The microbial degradation of phenol by pure cultures Bacillus cereus MTCC 9817 strain AKG1 and B. cereus MTCC 9818 strain AKG2 is studied in batch mode for several initial concentrations of phenol in the range of 100-2000 mg/L with an interval of 100mg/L. Degradation pathways are investigated at initial phenol concent...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2010.02.018
更新日期:2010-07-01 00:00:00
abstract::The aim of this work was to study the difference in properties of lignins, those (organosolv lignins (ORGLs, 12±3% yield and 93±5% mass balance) were isolated from diverse rice husk (RH) substrates using organosolv procedure (water:ethanol, H2SO4) carried out at 180°C for 1h. To identify the possible alterations in li...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2016.09.042
更新日期:2016-12-01 00:00:00
abstract::Food waste (FW) is traditionally disposed through landfills and incineration in China. Nowadays, there are some promising methods, such as anaerobic digestion (AD) or feeding and composting, which are being applied in pilot cities. However, the inherent characteristics of Chinese FW may be regarded as a double-edged s...
journal_title:Bioresource technology
pub_type: 杂志文章,评审
doi:10.1016/j.biortech.2018.03.102
更新日期:2018-07-01 00:00:00
abstract::High-solids anaerobic digestion (HSAD), a promising method with smaller reactor and less heating energy consumption, showed relatively lower digestion efficiency sometimes and higher tolerance to some inhibitors. To investigate the phenomena, the archaeal and bacterial communities in four anaerobic digesters treating ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2016.05.048
更新日期:2016-09-01 00:00:00
abstract::Seaweed Sargassum sp. biomass proved to be useful for the recovery of ionic copper from highly concentrated solutions simulating effluents from semiconductor production. In the case of solutions containing copper in the form of chloride, sulphate and nitrate salts, the best pH for the recovery of copper was 4.5. It wa...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2004.11.009
更新日期:2005-09-01 00:00:00
abstract::In this study, an inulinase-producing yeast strain 2F5 of Rhodosporidium toruloides was obtained. It was found that the yeast strain 2F5 could produce higher amount of oil from inulin and larger lipid bodies in its cells than any other yeast strains tested in this study. Under the optimal conditions, 62.14% (w/w) of l...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2014.03.038
更新日期:2014-06-01 00:00:00
abstract::This study aims to investigate the effects of microwaves, ultrasonic and alkaline pretreatments on olive pomace properties and its biomethane potential. Alkaline pretreatment was found to reduce lipid and fiber contents (especially lignin) and to increase soluble matter. The alkali pretreatment at a dose of 8% (w/w TS...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2019.122591
更新日期:2020-03-01 00:00:00
abstract::In this study, chemical compositions of fatty oils and bioactivity of crude extracts from Holotrichia diomphalia larvae as Chinese materia medica were investigated for the first time. The chemical compositions of the fatty oils were obtained by two different methods and determined by GC/MS. In total, the petroleum eth...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2008.03.010
更新日期:2008-11-01 00:00:00
abstract::The plant nutrients consumed in human society today are lost through the established wastewater treatment systems in industrialised countries as well as via insufficient or non-existent handling of sewage in the developing world. New sanitation systems have been designated to overcome this failure. The source separate...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2009.03.024
更新日期:2009-09-01 00:00:00