Abstract：
:Flax and sisal pulps were treated with two laccases (from Pycnoporus cinnabarinus, PcL and Trametes villosa, TvL, respectively), in the presence of different phenolic compounds (syringaldehyde, acetosyringone and p-coumaric acid in the case of flax pulp, and coniferaldehyde, sinapaldehyde, ferulic acid and sinapic acid in the case of sisal pulp). In most cases the enzymatic treatments resulted in increased kappa number of pulps suggesting the incorporation of the phenols into fibres. The covalent binding of these compounds to fibres was evidenced by the analysis of the treated pulps, after acetone extraction, by pyrolysis coupled with gas chromatography/mass spectrometry in the absence and/or in the presence of tetramethylammonium hydroxide (TMAH) as methylating agent. The highest extents of phenol incorporation were observed with the p-hydroxycinnamic acids, p-coumaric and ferulic acids. The present work shows for the first time the use of analytical pyrolysis as an effective approach to study fibre functionalization by laccase-induced grafting of phenols.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Aracri E,Fillat A,Colom JF,Gutiérrez A,Del Río JC,Martínez AT,Vidal Tdoi
10.1016/j.biortech.2010.05.080subject
Has Abstractpub_date
2010-11-01 00:00:00pages
8211-6issue
21eissn
0960-8524issn
1873-2976pii
S0960-8524(10)00938-7journal_volume
101pub_type
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