From paper mill waste to single cell oil: Enzymatic hydrolysis to sugars and their fermentation into microbial oil by the yeast Lipomyces starkeyi.

Abstract:

:Single cell oil (SCO) represents an outstanding alternative to both fossil sources and vegetable oils from food crops waste. In this work, an innovative two-step process for the conversion of cellulosic paper mill waste into SCO was proposed and optimised. Hydrolysates containing glucose and xylose were produced by enzymatic hydrolysis of the untreated waste. Under the optimised reaction conditions (Cellic® CTec2 25 FPU/g glucan, 48 h, biomass loading 20 g/L), glucose and xylose yields of 95 mol% were reached. The undetoxified hydrolysate was adopted as substrate for a batch-mode fermentation by the oleaginous yeast Lipomyces starkeyi. Lipid yield, content for single cell, production and maximum oil productivity were 20.2 wt%, 37 wt%, 3.7 g/L and 2.0 g/L/d respectively. This new generation oil, obtained from a negative value industrial waste, represents a promising platform chemical for the production of biodiesel, biosurfactants, animal feed and biobased plastics.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Di Fidio N,Dragoni F,Antonetti C,De Bari I,Raspolli Galletti AM,Ragaglini G

doi

10.1016/j.biortech.2020.123790

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

123790

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(20)31062-2

journal_volume

315

pub_type

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