Abstract:
:1-Alkenes are traditionally used as basic chemicals with great importance. Biosynthetic 1-alkenes also have the potential to serve as biofuels. In this study, we engineered a Pseudomonas lipolytic microbial platform for 1-alkene production using hydrophobic substrate as sole carbon source. Fatty acid decarboxylase UndA and UndB were cloned and expressed, which successfully produced 1-alkenes. Optimal culturing temperature and the interruption of competitive pathway were proven to be beneficial to 1-alkene synthesis. Chromosomal integration of UndB conferred 177.8 mg/L 1-alkenes (mainly 1-undecene) in lauric acid medium and 128.9 mg/L 1-alkenes (mainly 1-pentadecene) in palm oil medium. Thioesterase expression, adjustments of fatty acid degradation pathway and a second copy of UndB improved 1-alkene titer to 1102.6 mg/L using lauric acid and 778.4 mg/L using palm oil. All in all, this study offers the first demonstration of lipolytic microbial 1-alkene producing platform with highest reported 1-alkene product titer up to date.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Wang J,Yu H,Zhu Kdoi
10.1016/j.biortech.2018.04.119subject
Has Abstractpub_date
2018-09-01 00:00:00pages
172-179eissn
0960-8524issn
1873-2976pii
S0960-8524(18)30642-4journal_volume
263pub_type
杂志文章abstract::This paper reviews current knowledge of the production of organic acids by immobilized microorganisms with a simultaneous solubilization of rock phosphate in fermentation and soil conditions. The most widely applied methods are based on the passive immobilization in preformed porous carriers and entrapment of the micr...
journal_title:Bioresource technology
pub_type: 杂志文章,评审
doi:10.1016/s0960-8524(01)00017-7
更新日期:2001-09-01 00:00:00
abstract::The effect of enzyme loading under static and agitated conditions was investigated. Enzymatic hydrolysis of 10 w/v% de-lignified cellulose slurry such as filter paper, avicel and pulp was conducted under agitated (120 rpm) and static condition, and the enzyme loading ranging from 1.2 to 120 mg-protein/g-dry substrate....
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.06.104
更新日期:2012-10-01 00:00:00
abstract::The adaptive response of the fungus Blakeslea trispora to the oxidative stress induced by elevated dissolved oxygen concentrations during carotene production was investigated by measuring the specific activities of catalase (CAT) and superoxide dismutase (SOD) and the micromorphology of the fungus using a computerized...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2011.06.027
更新日期:2011-09-01 00:00:00
abstract::The dynamic response mechanism of Candidatus Accumulibacter clades to environmental factors in enhanced biological phosphorus removal (EBPR) was unclear. This study investigated the relationship between the transcriptional responses of Candidatus Accumulibacter clades and environmental dynamics. Results suggested that...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2020.123108
更新日期:2020-03-03 00:00:00
abstract::Solvent extraction of steam exploded lignocellulosic biomass may be a potential way to obtain antioxidative extracts and to enhance the enzymatic convertibility of the solid residue. Boiling solvent extraction (BSE) showed higher solid and phenolic yields than room temperature extraction. Solubilities of phenolics and...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.11.143
更新日期:2013-02-01 00:00:00
abstract::1,3-Propanediol (1,3-PDO) production from crude glycerol, a byproduct from biodiesel manufacturing, by Clostridium beijerinckii DSM 791 was studied with corn steep liquor as an inexpensive nitrogen source replacing yeast extract in the fermentation medium. A stable, long-term 1,3-PDO production from glycerol was demon...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2016.04.020
更新日期:2016-07-01 00:00:00
abstract::Cross-linked enzyme aggregates of poly-3-hydroxybutyrate (PHB) depolymerase from Streptomyces exfoliatus (PhaZ(Sex)-CLEAs) have been prepared. Acetone was used as the precipitating agent, while addition of bovine serum albumin (BSA) facilitated CLEAs formation. Conditions for enzyme precipitation and cross-linking hav...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2011.09.035
更新日期:2012-07-01 00:00:00
abstract::The objective of this work was to examine the feasibility of biogas production from the anaerobic co-digestion of herbal-extraction residues with swine manure. Batch and semi-continuous experiments were carried out under mesophilic anaerobic conditions. Batch experiments revealed that the highest specific biogas yield...
journal_title:Bioresource technology
pub_type: 杂志文章,收录出版
doi:10.1016/j.biortech.2011.03.093
更新日期:2011-06-01 00:00:00
abstract::Among many uses of rice straw, application of its biochar from pyrolysis to the soil is receiving greater interest for increased crop productivity and sequestration of CO2. This study investigated slow pyrolysis of rice straw at 300-700°C to characterize the yields and detailed composition of the biochar, bio-oil and ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2013.12.084
更新日期:2014-03-01 00:00:00
abstract::The traceability of conventional pollutants and 10 organic microcontaminants in the sludge line of a wastewater treatment plant (WWTP) was evaluated. The application of thermal hydrolysis (TH) as pre-treatment to anaerobic digestion (AD) or as inter-treatment (between two AD stages) was considered and compared with th...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2020.123028
更新日期:2020-02-15 00:00:00
abstract::Wood catalytic pyrolysis using calcium-based materials was studied in an auger reactor at 450°C. Two different catalysts, CaO and CaO·MgO were evaluated and upgraded bio-oils were obtained in both cases. Whilst acidity and oxygen content remarkable decrease, both pH and calorific value increase with respect to the non...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2014.03.146
更新日期:2014-06-01 00:00:00
abstract::Co-fermentation and simultaneous saccharification of rice hull hydrolysate (RHH) were investigated for the production of ethanol and xylitol by Saccharomyces cerevisiae, Spathaspora arborariae, or the combination of both. In bioreactor cultures under oxygen limitation, S. cerevisiae was capable of metabolizing glucose...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2013.05.123
更新日期:2013-09-01 00:00:00
abstract::Inhibitory lignocellulose hydrolysates were treated with the reducing agents dithionite and sulfite to achieve improved fermentability. Addition of these reducing agents (in the concentration range 5.0-17.5 mM) to enzymatic hydrolysates of spruce wood or sugarcane bagasse improved processes based on both SHF (simultan...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2010.08.037
更新日期:2011-01-01 00:00:00
abstract::Ibuprofen (IBU) is a non-steroidal drug that is classified as a trace organic compound (TrOC). A forward osmosis membrane bioreactor (FOMBR) has traditionally been a favored technology for wastewater treatment. In this study, the IBU degradation mechanism was clarified using an FOMBR. The results indicated that the av...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2020.124448
更新日期:2021-02-01 00:00:00
abstract::Fed-batch culture and the transformation conditions of Haematococcus pluvialis in a 5L photobioreactor were investigated. Methods of feeding model, low temperature at night and proper feeding time were used to increase both cell biomass and cell activity. Dry cell weight of 1.87 g/L which was 2.0-fold of batch culture...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2015.02.101
更新日期:2015-06-01 00:00:00
abstract::The aim of this work was to assess the environmental consequences of anaerobic mono- and co-digestion of pig manure to produce bio-energy, from a life cycle perspective. This included assessing environmental impacts and land use change emissions (LUC) required to replace used co-substrates for anaerobic digestion. Env...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.08.124
更新日期:2012-12-01 00:00:00
abstract::The influence of monoethanolamine (MEA) as a CO(2) absorbent on photoautotrophic culture of CO(2)-fixing microalgae was investigated. When 300 ppm MEA (4.92 mM) was added to blank culture medium, the dissolved inorganic carbon and the molar absorption ratio increased to 51.0mg/L and 0.34 mol CO2 = mol MEA, respectivel...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.06.010
更新日期:2012-09-01 00:00:00
abstract::Organosolv fractionation is a promising process to separate lignocellulosic biomass for the preparation of multiply products including biofuels, chemicals, and materials. This review presents the state of art of different processes applying alcohols and organic acids to treat lignocellulosic biomass for the production...
journal_title:Bioresource technology
pub_type: 杂志文章,评审
doi:10.1016/j.biortech.2015.10.004
更新日期:2016-01-01 00:00:00
abstract::The concentration of NaNO(3), MgSO(4) · 7H(2)O and proteose, in Chlorella protothecoides medium were optimized for algal biomass and lipid production by using response surface methodology with Box-Behnken design. The optimal concentrations were 0.45 g/L of NaNO(3), 6 mg/L of MgSO(4) · 7H(2)O, and 0.25 g/L of proteose ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.09.085
更新日期:2013-01-01 00:00:00
abstract::Non-productive enzyme adsorption onto lignin inhibits enzymatic hydrolysis of lignocellulosic biomass. Three cellobiohydrolases, Trichoderma reesei Cel7A (TrCel7A) and two engineered fusion enzymes, with distinctive modular structures and temperature stabilities were employed to study the effect of temperature on inhi...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2013.07.069
更新日期:2013-10-01 00:00:00
abstract::Optimum culture conditions which ease the synthesis of a novel exopolysaccharide (EPS) from a potent marine strain Streptococcus phocae was proposed in this study. The strain grows well at 35 °C, pH 7.0 and NaCl (2%) with lactose and yeast extract as best carbon and nitrogen sources. The maximum yield of EPS (11.75 an...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2010.12.118
更新日期:2011-04-01 00:00:00
abstract::In this study, a recent simple separation technique, three-phase partitioning (TPP), was used for concentrating microalgae Chlorella spp. for the first time. More than 91.7% of the biomass precipitated in the interlayer of the system in 10 min. Temperature, initial concentration and ratio of ethanol to dipotassium hyd...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2013.08.156
更新日期:2013-12-01 00:00:00
abstract::A photobioreactor (PBR) coupled with ultrafiltration (UF) system was developed with goals of microalgae cultivation, harvest, and membrane fouling control in the anaerobic digestion effluent purification. Firstly, three-sequencing batch PBRs were started-up with suspended Chlorella vulgaris (C. vulgaris, SCV), immobil...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2018.07.110
更新日期:2018-11-01 00:00:00
abstract::The effect of two enzymatic hydrolyzed molasses (EHM)-feeding strategies including constant-(CFR) and exponential-(EFR) feeding rate on canthaxanthin (CTX) biosynthesis by Dietzia natronolimnaea HS-1 fed-batch fermentation was studied. The results showed that the CFR of 7 ml/h with an EHM content of 45 g/l led to the ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2013.12.013
更新日期:2014-02-01 00:00:00
abstract::Three identical anoxic-aerobic membrane bioreactors (MBRs) were operated in parallel for 300 consecutive days for raw (R1), ozonated (R2) and catalytic ozonated (R3) biologically pretreated coal gasification wastewater (BPCGW) treatment. The results demonstrated that catalytic ozonation process (COP) applied asa pretr...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2017.09.029
更新日期:2017-12-01 00:00:00
abstract::Production of polyhydroxyalkanoates (PHAs) in activated sludge treating wastewater represents an economical and environmental promising alternative to pure culture fermentations. A process for production of PHA from a paper mill wastewater was examined at laboratory scale. The three stage process examined consisted of...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2007.01.020
更新日期:2008-02-01 00:00:00
abstract::Municipal solid waste (MSW) represents an attractive cellulosic resource for sustainable fuel production. However, its heterogeneity is the major barrier to efficient conversion to biofuels. MSW paper mix was generated and blended with corn stover (CS). It has been shown that both of them can be efficiently pretreated...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2015.02.087
更新日期:2015-06-01 00:00:00
abstract::(2S,3S)-2,3-Butanediol ((2S,3S)-2,3-BD) is a crucial chiral compound that acts as an excellent building block in asymmetric synthesis of highly valuable chiral compounds. However, the low concentration and optical purity of (2S,3S)-2,3-BD produced in previous studies limited its applications. In the present work, the ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2011.08.097
更新日期:2012-07-01 00:00:00
abstract::Statistical optimization designs were used to optimize the phenol degradation using Chlorella pyrenoidosa. The important factor influencing phenol degradation was identified by two-level Plackett-Burman Design (PBD) with five factors. PBD determined the following three factors as significant for phenol degradation viz...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2016.01.138
更新日期:2016-05-01 00:00:00
abstract::A novel bacterium, Ochrobactrum sp. T, capable of simultaneous debromination and aerobic mineralization of tetrabromobisphenol-A (TBBPA), was isolated from a sludge sample collected from an electronic-waste recycling site. The bacterium exhibited maximal debrominase activity at pH 6.5, 35 °C, and 200 rpm in Luria-Bert...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2011.06.080
更新日期:2011-10-01 00:00:00