Employing metabolic engineered lipolytic microbial platform for 1-alkene one-step conversion.

Abstract:

:1-Alkenes are traditionally used as basic chemicals with great importance. Biosynthetic 1-alkenes also have the potential to serve as biofuels. In this study, we engineered a Pseudomonas lipolytic microbial platform for 1-alkene production using hydrophobic substrate as sole carbon source. Fatty acid decarboxylase UndA and UndB were cloned and expressed, which successfully produced 1-alkenes. Optimal culturing temperature and the interruption of competitive pathway were proven to be beneficial to 1-alkene synthesis. Chromosomal integration of UndB conferred 177.8 mg/L 1-alkenes (mainly 1-undecene) in lauric acid medium and 128.9 mg/L 1-alkenes (mainly 1-pentadecene) in palm oil medium. Thioesterase expression, adjustments of fatty acid degradation pathway and a second copy of UndB improved 1-alkene titer to 1102.6 mg/L using lauric acid and 778.4 mg/L using palm oil. All in all, this study offers the first demonstration of lipolytic microbial 1-alkene producing platform with highest reported 1-alkene product titer up to date.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Wang J,Yu H,Zhu K

doi

10.1016/j.biortech.2018.04.119

subject

Has Abstract

pub_date

2018-09-01 00:00:00

pages

172-179

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(18)30642-4

journal_volume

263

pub_type

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