Abstract：
:A rapid empirical assay is presented for assessing the phenotypic stability of continuous cultures of recombinant bacteria containing transposed pdc and adh genes for ethanol production. The method measures spectrophotometrically the rate of colour formation when cells oxidize added ethanol to acetaldehyde in the presence of Schiff's reagent. During chemostat cultures of the recombinant ethanologen Escherichia coli KO11 on 20 g/l glucose, assay activities were stable and high at ca 8 x 10(-4) DeltaOD(540)/(s.OD(550)), reflecting the high, stable ethanol yield (ca 95%). On 20 g/l and 50 g/l xylose, ethanol yields declined rapidly to about 60% and this was closely mirrored by the assay activities which fell to ca 1.5 DeltaOD(540)/(s.OD(550)), only slightly higher than those measured for the parent strain. Typically taking only about an hour to perform, the assay provides a faster means of gauging the phenotypic stability of ethanol production than is possible by conventional methods.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zhou B,Martin GJ,Pamment NBdoi
10.1016/j.biortech.2009.01.013subject
Has Abstractpub_date
2009-06-01 00:00:00pages
2823-7issue
11eissn
0960-8524issn
1873-2976pii
S0960-8524(09)00039-Xjournal_volume
100pub_type
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