Induction of triacylglycerol production in Chlamydomonas reinhardtii: comparative analysis of different element regimes.

Abstract:

:In this study, impacts of different element absence (nitrogen, sulfur, phosphorus and magnesium) and supplementation (nitrogen and zinc) on element uptake and triacylglycerol production was followed in wild type Chlamydomonas reinhardtii CC-124 strain. Macro- and microelement composition of C. reinhardtii greatly differed under element regimes studied. In particular, heavy metal quotas of the microalgae increased strikingly under zinc supplementation. Growth was suppressed, cell biovolume, carbohydrate, total neutral lipid and triacylglycerol levels increased when microalgae were incubated under these element regimes. Most of the intracellular space was occupied by lipid bodies under all nutrient starvations, as observed by confocal microscopy and transmission electron micrographs. Results suggest that sulfur, magnesium and phosphorus deprivations are superior to nitrogen deprivation for the induction triacylglycerol production in C. reinhardtii. On the other hand, FAME profiles of the nitrogen, sulfur and phosphorus deprived cells were found to meet the requirements of international standards for biodiesel.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Çakmak ZE,Ölmez TT,Çakmak T,Menemen Y,Tekinay T

doi

10.1016/j.biortech.2013.12.093

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

379-87

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(13)01933-0

journal_volume

155

pub_type

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