Abstract：
:The Bxl5-gene encoding a GH3 glycoside hydrolase of Chrysosporium lucknowense C1 was successfully cloned, the homologous recombinant product was secreted, purified and characterized. Bxl5 (120 ± 5 kDa) was able to hydrolyze low molecular weight substrates and polysaccharides containing β-glucosidic as well as β-xylosidic residues. The K(m) and V(max)/E values were found to be 0.3mM and 88 s(-1) on p-nitrophenyl-β-d-glucopyranoside (PNPG), and 13.5mM and 1.8s(-1) on p-nitrophenyl-β-d-xylopyranoside (PNPX). Optimal pH and temperature for Bxl5 were 4.6 and 75°C for the PNPG hydrolysis, and 5.0-5.5 and 70°C for PNPX hydrolysis. The enzyme was quite stable when incubated at elevated temperatures up to 65°C. Bxl5 hydrolyzes polymeric β-glucans by the exo-mechanism allowing their complete conversion to d-glucose and is effective for xylan hydrolysis in combination with endo-acting xylan-degrading enzymes. The enzyme seems to be a very promising for bioconversion purposes.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Dotsenko GS,Sinitsyna OA,Hinz SW,Wery J,Sinitsyn APdoi
10.1016/j.biortech.2012.02.105subject
Has Abstractpub_date
2012-05-01 00:00:00pages
345-9eissn
0960-8524issn
1873-2976pii
S0960-8524(12)00356-2journal_volume
112pub_type
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