Feruloyl esterases from Schizophyllum commune to treat food industry side-streams.

Abstract:

:Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000UL(-1). The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Nieter A,Kelle S,Linke D,Berger RG

doi

10.1016/j.biortech.2016.08.045

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

38-46

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(16)31173-7

journal_volume

220

pub_type

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