Abstract:
:A strain of Bacillus licheniformis 8-37-0-1 with high exopolysaccharide (EPS) production ability was isolated and identified based on morphological and physiological characteristics and phylogenetic analysis of 16S rDNA sequences. A new type of EPS was isolated from the strain fermentation broth by enzymolysis, isopropanol precipitation, anion-exchange, and gel-filtration chromatography. The new EPS was determined as homogeneous, with a molecular weight of 2.826 x 10(4), as determined by High-Performance Size-Exclusion Chromatography Multi-Angle Laser Light Scattering analysis. Its structural characteristics were investigated and elucidated by methylation analysis, partial acid hydrolysis, gas-liquid chromatography mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance spectroscopy. Based on obtained data, the EPS was found to be a levan containing a (2-->6)-linked backbone with a single beta-d-fructose at the C-1 position every seven residue, on average, along the main chain. Preliminary in vitro tests revealed that EPS could significantly stimulate the proliferation of spleen lymphocyte.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Liu C,Lu J,Lu L,Liu Y,Wang F,Xiao Mdoi
10.1016/j.biortech.2010.01.151subject
Has Abstractpub_date
2010-07-01 00:00:00pages
5528-33issue
14eissn
0960-8524issn
1873-2976pii
S0960-8524(10)00317-2journal_volume
101pub_type
杂志文章abstract::Anaerobic digestion (AD) of vinegar residue was investigated in continuously stirred tank reactor (CSTR). The influence of organic loading rate (OLR) and effluent recirculation on AD performance of vinegar residue was tested. Five OLRs, 1.0, 1.5, 2.0, 2.5, and 3.0 g(vs) L(-1) d(-1), were used. The highest volumetric m...
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pub_type: 杂志文章,评审
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