Identification and characterization of microorganisms from earthworm viscera for the conversion of fish wastes into liquid fertilizer.

Abstract:

:Five bacteria isolated from earthworm viscera and identified as Brevibacillus agri, Bacillus cereus, Bacillus licheniformis, and Brevibacillus parabrevis by 16S rRNA sequencing were employed in the conversion of fish wastes generated from a restaurant specializing in sliced raw fish into fertilizer. Within 120h after inoculation of autoclaved fish waste with 5.15 x 10(5) CFU ml(-1) mixed isolates, the amount of dry sludge decreased from 29.4 to 0.2g, the pH changed from 7.05 to 5.70, and the cell number reached 6.45 x 10(5) CFU ml(-1). Analyses of an 84-h culture of inoculated fish waste indicated low phytotoxicity in a seed germination test, an amino acid content of 5.71 g 100 g(-1), a low concentration of heavy metals (Pb, As, Cd, Hg, Cr, Cu, Ni and Zn), and a N/P/K level of 2.33%. Therefore the converted fish waste has the potential for use as liquid fertilizer, although the low NPK level is a concern. This is the first demonstration of the reutilization of fish wastes as a liquid fertilizer.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Kim JK,Dao VT,Kong IS,Lee HH

doi

10.1016/j.biortech.2010.02.001

subject

Has Abstract

pub_date

2010-07-01 00:00:00

pages

5131-6

issue

14

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(10)00272-5

journal_volume

101

pub_type

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