Decolorizing kinetics of a recombinant Escherichia coli SS125 strain harboring azoreductase gene from Bacillus latrosporus RRK1.

Abstract:

:PCR amplified product containing gene responsible for dye decolorization was cloned and expressed in Escherichia coli. The resulting recombinant strain E. coli SS125 decolorized 200mg/l azo dye (Remazol Red) at 30 degrees C at 255 mg cell/l/h, while the host E. coli (DH5 alpha) had no color removal ability. The dependence of the decolorization rate on initial dye concentration and the maximum rate occurred with the dye at 100 mg l(-1). The decolorization rate of E. coli SS125 was optimal at 37-45 degrees C. Aeration strongly-inhibited the decolorization, but decolorization occurred effectively under static and anaerobic incubation conditions. The E. coli SS125 strain also exhibited excellent stability during reported batch operation.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Sandhya S,Sarayu K,Uma B,Swaminathan K

doi

10.1016/j.biortech.2007.05.027

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

2187-91

issue

7

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(07)00448-8

journal_volume

99

pub_type

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