Improved production of 2,5-furandicarboxylic acid by overexpression of 5-hydroxymethylfurfural oxidase and 5-hydroxymethylfurfural/furfural oxidoreductase in Raoultella ornithinolytica BF60.

Abstract:

:2,5-Furandicarboxylic acid (FDCA) is a promising bio-based building block and can be produced by biotransformation of 5-hydroxymethylfurfural (HMF). To improve the FDCA production, two genes-one encoding HMF oxidase (HMFO; from Methylovorus sp. strain MP688) and another encoding for HMF/Furfural oxidoreductase (HmfH; from Cupriavidus basilensis HMF14)-were introduced into Raoultella ornithinolytica BF60. The FDCA production in the engineered whole-cell biocatalyst increased from 51.0 to 93.6mM, and the molar conversion ratio of HMF to FDCA increased from 51.0 to 93.6%.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Yuan H,Li J,Shin HD,Du G,Chen J,Shi Z,Liu L

doi

10.1016/j.biortech.2017.08.166

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

1184-1188

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(17)31480-3

journal_volume

247

pub_type

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