Abstract:
:2,5-Furandicarboxylic acid (FDCA) is a promising bio-based building block and can be produced by biotransformation of 5-hydroxymethylfurfural (HMF). To improve the FDCA production, two genes-one encoding HMF oxidase (HMFO; from Methylovorus sp. strain MP688) and another encoding for HMF/Furfural oxidoreductase (HmfH; from Cupriavidus basilensis HMF14)-were introduced into Raoultella ornithinolytica BF60. The FDCA production in the engineered whole-cell biocatalyst increased from 51.0 to 93.6mM, and the molar conversion ratio of HMF to FDCA increased from 51.0 to 93.6%.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Yuan H,Li J,Shin HD,Du G,Chen J,Shi Z,Liu Ldoi
10.1016/j.biortech.2017.08.166subject
Has Abstractpub_date
2018-01-01 00:00:00pages
1184-1188eissn
0960-8524issn
1873-2976pii
S0960-8524(17)31480-3journal_volume
247pub_type
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