Thermostability, pH stability and dye degrading activity of a bacterial laccase are enhanced in the presence of Cu2O nanoparticles.

Abstract:

:The present study relates to a nanotechnology enabled method in which purified laccase from Escherichia coli AKL2 was supplemented with 100 μM copper oxide nanoparticles (Cu(2)O) (NP-laccase). The activity, half life and stability of NP-laccase were enhanced by 4, 42 and 36-fold respectively at high temperature (80 °C) and also over a wide range of pH (4-12) than laccase (in the presence of 0.18 mM CuSO(4)). Thermodynamic analysis of the nanoparticle-induced enzyme stability revealed an enhanced entropy-enthalpy compensation at 80 °C, which reflected the maintenance of its native structure. This was further supported by CD studies. The enhanced activity and thermostability of NP-laccase can be utilized for efficient decolorisation of dyes (both phenolic and azo).

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Mukhopadhyay A,Dasgupta AK,Chakrabarti K

doi

10.1016/j.biortech.2012.09.087

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

25-36

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(12)01437-X

journal_volume

127

pub_type

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