Butanol production by Clostridium beijerinckii. Part I: use of acid and enzyme hydrolyzed corn fiber.

Abstract:

:Fermentation of sulfuric acid treated corn fiber hydrolysate (SACFH) inhibited cell growth and butanol production (1.7+/-0.2g/L acetone butanol ethanol or ABE) by Clostridium beijerinckii BA101. Treatment of SACFH with XAD-4 resin removed some of the inhibitors resulting in the production of 9.3+/-0.5 g/L ABE and a yield of 0.39+/-0.015. Fermentation of enzyme treated corn fiber hydrolysate (ETCFH) did not reveal any cell inhibition and resulted in the production of 8.6+/-1.0 g/L ABE and used 24.6g/L total sugars. ABE production from fermentation of 25 g/L glucose and 25 g/L xylose was 9.9+/-0.4 and 9.6+/-0.4 g/L, respectively, suggesting that the culture was able to utilize xylose as efficiently as glucose. Production of only 9.3+/-0.5 g/L ABE (compared with 17.7 g/L ABE from fermentation of 55 g/L glucose-control) from the XAD-4 treated SACFH suggested that some fermentation inhibitors may still be present following treatment. It is suggested that inhibitory components be completely removed from the SACFH prior to fermentation with C. beijerinckii BA101. In our fermentations, an ABE yield ranging from 0.35 to 0.39 was obtained, which is higher than reported by the other investigators.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Qureshi N,Ezeji TC,Ebener J,Dien BS,Cotta MA,Blaschek HP

doi

10.1016/j.biortech.2007.09.087

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

5915-22

issue

13

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(07)00866-8

journal_volume

99

pub_type

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