Abstract：
:Isolation of fiber concentrate enriched in β-glucan from barley flour via air currents assisted particle separation (ACAPS) generates an underutilized by-product stream, starch concentrate. Since barley starch concentrate (BSC) is depleted in soluble fibre, we examined the enzyme requirements for its hydrolysis and subsequent fermentation. Lower doses of a common raw starch hydrolyzing enzyme (STARGEN™ 002) effectively hydrolyzed BSC, achieving similar hydrolysis kinetics to the wheat benchmark. Hydrolysis of BSC did not require further enzyme supplementation, which is required for optimal wheat hydrolysis. This likely resulted from the smaller particle size of BSC relative to wheat feedstocks. Interestingly, simultaneous saccharification and fermentation of BSC using a 0.25X dose of STARGEN™ 002 alone enabled efficient ethanol production, though a requirement for phosphorus supplementation was identified. This study proposes a biorefining strategy that supports the generation of a value-added co-product, starch concentrate, while significantly reducing the enzyme requirements for bioethanol production.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Lu Y,Chae M,Vasanthan T,Bressler DCdoi
10.1016/j.biortech.2020.122942subject
Has Abstractpub_date
2020-05-01 00:00:00pages
122942eissn
0960-8524issn
1873-2976pii
S0960-8524(20)30211-Xjournal_volume
303pub_type
杂志文章abstract：:Biological nitrogen fixation is a major pathway in ecosystems. This study investigated the effects of adding Cu at different levels (0, 200, and 2000 mg kg-1) on the diazotroph community during swine manure composting. Quantitative PCR and high-throughput sequencing were used to analyze the abundances of diazotrophs a...
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