Abstract：
:Dry dilute acid pretreatment (DDAP) is a promising method for lignocellulose bioconversion, although inhibitors generated during the pretreatment impede the fermentation severely. We developed the simultaneous saccharification and co-fermentation (SScF) of DDAP pretreated biomass at high solid loading using xylose fermenting Saccharomyces cerevisiae, SyBE005. Effect of temperature on SScF showed that ethanol yield at 34°C was 10.2% higher than that at 38°C. Ethanol concentration reached 29.5 g/L at 15% (w/w) dry matter loading, while SScF almost ceased at the beginning at 25% (w/w) dry matter loading of DDAP pretreated corn stover. According to the effect of the diluted hydrolysate on the fermentation of strain SyBE005, a fed-batch mode was developed for the SScF of DDAP pretreated corn stover with 25% dry matter loading without detoxification, and 40.0 g/L ethanol was achieved. In addition, high yeast inoculation improved xylose utilization and the final ethanol concentration reached 47.2 g/L.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zhu JQ,Qin L,Li WC,Zhang J,Bao J,Huang YD,Li BZ,Yuan YJdoi
10.1016/j.biortech.2015.08.140subject
Has Abstractpub_date
2015-12-01 00:00:00pages
39-46eissn
0960-8524issn
1873-2976pii
S0960-8524(15)01256-0journal_volume
198pub_type
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