Abstract：
:Vibrational wave treatment has been used to increase proliferation of microalgae. When directly applied at large scale, however, turbulence can offset positive effects of vibration on microalgae proliferation. Moreover, severe hydrodynamic shear fields in the bioreactor decrease cell viability that detrimentally influence maximum yieldable biomass. In this study, vibration pretreatment (between 10-30 Hz and 0.15-0.45 G) was used to prime the cells for enhanced biomass. When exposed to 10 Hz at 0.15 G for 72 h and inoculated in baffled flasks of large shear fields (0.292 Pa for the average wall shear force (aveWSF) and 184 s-1 for the average shear strain rate (aveSSR)), microalgae showed 27% increase in biomass as well as 39% increase in corresponding amount of heterologous protein (i.e. GFP-3HA). Our results show that stress primed microalgae with vibrations can lead to improved proliferation that results in increased biomass production at industrial scale bioprocesses.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Paik SM,Jin E,Sim SJ,Jeon NLdoi
10.1016/j.biortech.2018.01.108subject
Has Abstractpub_date
2018-04-01 00:00:00pages
340-346eissn
0960-8524issn
1873-2976pii
S0960-8524(18)30122-6journal_volume
254pub_type
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